pSIVA Apoptosis Detection Kit Summary
| Description |
Polarity Sensitive Indicator of Viability (pSIVA(TM) is an Annexin based, polarity sensitive probe for the spatiotemporal or kinetic analysis of apoptosis and other forms of cell death. pSIVA(TM) binding is reversible, enabling researchers to detect transient phosphatidylserine (PS) exposure which is associated with normal physiological processes as well as with reversible or rescuable apoptosis cell death events. pSIVA(TM) is conjugated to IANBD, a polarity sensitive dye that fluoresces only when pSIVA(TM) is bound to the cell membrane. pSIVA's membrane-bound dependent fluorescence and reversible binding properties are a technological leap for detecting PS exposure and offer additional information on the apoptosis pathway and cell survival compared to Annexin V conjugates. Annexin V binding is nonreversible. |
| Kit Type |
Kit |
Applications/Dilutions
| Dilutions |
- Immunocytochemistry/ Immunofluorescence
- In vitro assay
- In vivo assay
- Live Imaging Microscopy
|
| Application Notes |
See attached PDF protocol for specific usage information. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24162663). use in ICC/IF, in vitro , and in vivo. reported in scientific literature (PMID: 19966809). |
| Reviewed Applications |
Read 1 Review rated 5 using
NBP2-29382 in the following applications:
|
|
| Publications |
Read Publications using
NBP2-29382 in the following applications:
|
|
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 24999049). Human reactivity reported in scientific literature (PMID: 25692494). Rat reactivity reported in scientific literature (PMID: 19966809).
Packaging, Storage & Formulations
Kit Components
|
Components
|
- Propidium Iodide Staining Solution (500 uL)
- pSIVA-IANBD (200 uL)
|
Notes
pSIVA is protected under patent number: 8,541,549. This is the same kit which was sold as pSIVA(TM) - IANBD Apoptosis/Viability Microscopy Set [Catalog Number IMG-6701K] by Imgenex - now a part of Novus Biologicals, a Bio-Techne brand.
Alternate Names for pSIVA Apoptosis Detection Kit
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
Product General Protocols
View specific protocols for pSIVA Apoptosis Detection Kit (NBP2-29382):
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for pSIVA Apoptosis Detection Kit (NBP2-29382). (Showing 1 - 2 of 2 FAQs).
-
Our end user is working on tissues (epithelium of imaginal disk from drosophila leg). The tissues are kept growing on a culture medium and they manage to follow apoptosis properly with orange acrylin but not with annexin V because of background. Did you ever test any of your pSIVA apoptosis kits (NBP2-29382, NBP2-29611) on tissue culture instead of cell culture ? Is there a chance the proble will work on such an application ? Would you be able to provide a sample for this application ? As for the other samples, I'm still waiting on customer feedback and I'll keep you posted.
- Yes, pSIVA has been used in tissue applications as denoted on the publication link. Specifically, I would encourage the research to look at the following publications from the link: 1. Diurnal, localized exposure of phosphatidylserine by rod outer segment tips in wild-type but not Itgb5-/- or Mfge8-/- mouse retina. Ruggiero L, MP Connor, J Chen, R Langen, SC Finnemann. PNAS 109:8145-4148 (2012). Live tissue imaging (mouse retina): Figs 4, 5. S4. pSIVA-IANBD was added to dissected live mouse retina and shown to label the tips of photoreceptor outer segments (POS). > The results suggested that phosphatidylserine (PS) exposure is specific to the POS surface. Furthermore, enhanced PS exposure preceded rod shedding and phagocytosis, suggesting that surface PS exposure promotes these processes. 2. Diurnal photoreceptor outer segment shedding: contributions of the RPE. Finnemann SC, L Ruggiero, MP Connor. Invest Opthalmol Vis Sci 53:4327 (2012). Live Imaging: mouse retina. pSIVA was used quantify the surface exposure of phosphotidylserine on the distal rod photoreceptor outer segments. 3. Characterization of cell cycle modifications induced by electric pulses in human corneal endothelium. Thi MH, Z He, N Campolmi, S Piselli, P Gain, M Peoc'H, JM Dumolllard, S Acquart, O Garraud, G Thuret. Acta Opthalmologica 90:s249 (2012). Live tissue imaging (human cornea organ cultures): pSIVA-IANBD was used to assess cell death following gene electrotransfer into corneal endothelial cells. > 3. Engineering a polarity-sensitive biosensor for time-lapse imaging of apoptotic processess and degeneration. Kim YE, J Chen, JR Chan, R Langen. Nature Methods 7:67-73 (2010a). Real-time live-cell imaging and time-lapse microscopy of apoptosis: Fig 2 (COS-7 cells), Fig 3 (rat neuronal degeneration), Fig 4 (rat axonal degeneration), Fig 5 (rescue of rat neuronal degeneration as visualized by pSIVA. (this was also used in a rat nerve injury tissue model) Please watch the pSIVA webinar on our website for additional information about pSIVA. The webinar is referred to March 25, 2014: Real-Time Tracking of Cell Death Webinar. We would certainly be pleased to offer any of your end user research customers a sample who is willing to provide a short explanation about what they are planning to do, their timeframe, and provide feedback about how it worked for them. For any samples, please specify if you
-
How many tests can you perform with this kit?
- 100 tests for the full sized kit and 25 tests for the 1 sample sized kit.
Additional pSIVA Apoptosis Detection Products
Blogs on pSIVA Apoptosis Detection
![Live Imaging Microscopy: Polarity Sensitive Indicator of Viability Apoptosis Microscopy Kit [NBP2-29382] - Rescue of dying neurons: NGF withdrawal leads to cell death and pSIVA-IANBD(TM) fluorescence. Addition of NGF eventually rescues some neurons as shown by the loss of pSIVA-IANBD(TM) fluorescence (30h boxes).](http://images.novusbio.com/fullsize/Polarity-Sensitive-Indicator-of-Viability-Apoptosis-Microscopy-Kit-Live-Imaging--Microscopy-NBP2-29382-img0005.jpg "Live Imaging Microscopy: Polarity Sensitive Indicator of Viability Apoptosis Microscopy Kit [NBP2-29382] - Rescue of dying neurons: NGF withdrawal leads to cell death and pSIVA-IANBD(TM) fluorescence. Addition of NGF eventually rescues some neurons as shown by the loss of pSIVA-IANBD(TM) fluorescence (30h boxes).")
![Live Imaging Microscopy: Polarity Sensitive Indicator of Viability Apoptosis Microscopy Kit [NBP2-29382] - Rescue of dying neurons. NGF withdrawal leads to cell death and pSIVA-IANBD(TM) fluorescence. Correlation of fluorescence and phase microscopy shows that NGF rescued the neurons as shown by the loss of pSIVA-IANBD(TM) fluorescence at 30h.](http://images.novusbio.com/fullsize/Polarity-Sensitive-Indicator-of-Viability-Apoptosis-Microscopy-Kit-Live-Imaging--Microscopy-NBP2-29382-img0006.jpg "Live Imaging Microscopy: Polarity Sensitive Indicator of Viability Apoptosis Microscopy Kit [NBP2-29382] - Rescue of dying neurons. NGF withdrawal leads to cell death and pSIVA-IANBD(TM) fluorescence. Correlation of fluorescence and phase microscopy shows that NGF rescued the neurons as shown by the loss of pSIVA-IANBD(TM) fluorescence at 30h.")
![Live Imaging Microscopy: Polarity Sensitive Indicator of Viability Apoptosis Microscopy Kit [NBP2-29382] - Quantitative analysis of pSIVA-IANBD™ fluorescence is rescued and NGF deprived neuronal cultures. pSIVA-IANBD(TM) fluorescence from 3 areas of the culture shows that NGF rescue (A) of dying cells was associated with loss of fluorescence whereas unrescued cells (B) continued to fluoresce.](http://images.novusbio.com/fullsize/Polarity-Sensitive-Indicator-of-Viability-Apoptosis-Microscopy-Kit-Live-Imaging--Microscopy-NBP2-29382-img0007.jpg "Live Imaging Microscopy: Polarity Sensitive Indicator of Viability Apoptosis Microscopy Kit [NBP2-29382] - Quantitative analysis of pSIVA-IANBD™ fluorescence is rescued and NGF deprived neuronal cultures. pSIVA-IANBD(TM) fluorescence from 3 areas of the culture shows that NGF rescue (A) of dying cells was associated with loss of fluorescence whereas unrescued cells (B) continued to fluoresce.")
![Live Imaging Microscopy: Polarity Sensitive Indicator of Viability Apoptosis Microscopy Kit [NBP2-29382] - Rescue of dying photoreceptors in dystrophic rat neural retina. Image from verified customer review.](http://images.novusbio.com/fullsize/Polarity-Sensitive-Indicator-of-Viability-Apoptosis-Microscopy-Kit-Live-Imaging--Microscopy-NBP2-29382-img0008.jpg "Live Imaging Microscopy: Polarity Sensitive Indicator of Viability Apoptosis Microscopy Kit [NBP2-29382] - Rescue of dying photoreceptors in dystrophic rat neural retina. Image from verified customer review.")
