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Plexin B1 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: Plexin B1 Antibody [NBP1-87782] - Staining in human gallbladder and skeletal muscle tissues using anti-PLXNB1 antibody. Corresponding PLXNB1 RNA-seq data are ...read more
Western Blot: Plexin B1 Antibody [NBP1-87782] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line RT-4
Immunohistochemistry-Paraffin: Plexin B1 Antibody [NBP1-87782] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: Plexin B1 Antibody [NBP1-87782] - Staining of human gall bladder shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Plexin B1 Antibody [NBP1-87782] - Staining of human fallopian tube shows moderate cytoplasmic/ membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Plexin B1 Antibody [NBP1-87782] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules and moderate membranous/ cytoplasmic positivity in cells in glomeruli.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Plexin B1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PGDNECVMELEGLEVVVEARVECEPPPDTQCHVTCQQHQLSYEALQPELRVGLFLRRAGRLRVDSAEGLHVVLYDCSVGHGDCSRCQTAMPQYGCVWC
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLXNB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Plexin B1 Protein (NBP1-87782PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (82%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Plexin B1 Antibody

  • Plexin B1
  • PLXN5
  • PLXNB1
  • SEP
  • Transmembrane Protein SEP

Background

Receptor for SEMA4D. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol PLXNB1