We are looking into protein kinase C-alpha antibodies for immunohistochemistry on formalin fixed, paraffin embedded tissue. I see that you have many antibodies, including several monoclonals. I wanted to confirm with you that the following antibodies are specific for C-alpha. NB600-201=MC5 (an older paper suggested that this might react with alpha, beta, gamm), NB110-57356=Y124, NB110-57353=Y143. Also, if you have an comparator data or feedback regarding PKC-alpha antibodies for IHC on FFPE, that would be very helpful. We would like to pick 1 antibody that is easy to titer and robust, so that we can move forward with our studies.

Name: PKC alpha Antibody (MC5) [Janelia Fluor&#174; 646]
Brand: Novus Biologicals
SKU: NB600-201JF646
Price: 499 USD

Question

We are looking into protein kinase C-alpha antibodies for immunohistochemistry on formalin fixed, paraffin embedded tissue.  I see that you have many antibodies, including several monoclonals.  I wanted to confirm with you that the following antibodies are specific for C-alpha.  NB600-201=MC5 (an older paper suggested that this might react with alpha, beta, gamm), NB110-57356=Y124, NB110-57353=Y143.  Also, if you have an comparator data or feedback regarding PKC-alpha antibodies for IHC on FFPE, that would be very helpful. We would like to pick 1 antibody that is easy to titer and robust, so that we can move forward with our studies.

Accepted Answer

We have never heard any feedback that these cross-react with other PKC proteins. If you have seen data that MC5 may cross-react, then I would consider avoiding this antibody if you are concerned. The immunogens for NB110-57356 and NB110-57353 come from the N- and C-terminals, which is where the PKC proteins vary the most, so these are not expected to cross-react and we have had no feedback or testing data that suggests that they do. I would recommend NB110-57356, since it has been published with. You may want to review the published data to determine if it seems this antibody will suit your needs.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, Po, Bv, Ca, Ch, Fi, Pm, Pm, Rb, Sh, ZeSpecies Glossary
Applications	WB, ELISA, Flow, Func, ICC/IF, IHC, IP
Clone	MC5
Clonality	Monoclonal
Host	Mouse
Conjugate	Janelia Fluor 646

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB600-201AF350

Alexa Fluor 405 NB600-201AF405

Alexa Fluor 488 NB600-201AF488

Alexa Fluor 532 NB600-201AF532

Alexa Fluor 594 NB600-201AF594

Alexa Fluor 647 NB600-201AF647

Alexa Fluor 700 NB600-201AF700

Alexa Fluor 750 NB600-201AF750

Allophycocyanin NB600-201APC

Allophycocyanin/Cy7 NB600-201APCCY7

Biotin NB600-201B

CoraFluor 1 NB600-201CL1

DyLight 350 NB600-201UV

DyLight 405 NB600-201V

DyLight 488 NB600-201G

DyLight 550 NB600-201R

DyLight 594 NB600-201DL594

DyLight 650 NB600-201C

DyLight 680 NB600-201FR

DyLight 755 NB600-201IR

FITC NB600-201F

HRP NB600-201H

Janelia Fluor 525 NB600-201JF525

Janelia Fluor 549 NB600-201JF549

Janelia Fluor 585 NB600-201JF585

Janelia Fluor 635 NB600-201JF635

Janelia Fluor 669 NB600-201JF669

PE NB600-201PE

PE/Atto594 NB600-201PEATT594

PE/Cy5.5 NB600-201PECY55

PE/Cy7 NB600-201PECY7

PerCP NB600-201PCP

Unconjugated NB600-201

mFluor Violet 450 SE NB600-201MFV450

mFluor Violet 500 SE NB600-201MFV500

mFluor Violet 610 SE NB600-201MFV610

Catalog# & Formulation	Size	Price
Standard Formulation NB600-201

Azide and BSA Free NBP2-80911

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-201AF350
Alexa Fluor 405	NB600-201AF405
Alexa Fluor 488	NB600-201AF488
Alexa Fluor 532	NB600-201AF532
Alexa Fluor 594	NB600-201AF594
Alexa Fluor 647	NB600-201AF647
Alexa Fluor 700	NB600-201AF700
Alexa Fluor 750	NB600-201AF750
Allophycocyanin	NB600-201APC
Allophycocyanin/Cy7	NB600-201APCCY7
Biotin	NB600-201B
CoraFluor 1	NB600-201CL1
DyLight 350	NB600-201UV
DyLight 405	NB600-201V
DyLight 488	NB600-201G
DyLight 550	NB600-201R
DyLight 594	NB600-201DL594
DyLight 650	NB600-201C
DyLight 680	NB600-201FR
DyLight 755	NB600-201IR
FITC	NB600-201F
HRP	NB600-201H
Janelia Fluor 525	NB600-201JF525
Janelia Fluor 549	NB600-201JF549
Janelia Fluor 585	NB600-201JF585
Janelia Fluor 635	NB600-201JF635
Janelia Fluor 669	NB600-201JF669
PE	NB600-201PE
PE/Atto594	NB600-201PEATT594
PE/Cy5.5	NB600-201PECY55
PE/Cy7	NB600-201PECY7
PerCP	NB600-201PCP
Unconjugated	NB600-201
mFluor Violet 450 SE	NB600-201MFV450
mFluor Violet 500 SE	NB600-201MFV500
mFluor Violet 610 SE	NB600-201MFV610

PKC alpha Antibody (MC5) [Janelia Fluor® 646]

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Immunogen	Purified bovine brain Protein Kinase C
Specificity	This antibody is capable of detecting PKC beta as well.
Isotype	IgG2a Kappa
Clonality	Monoclonal
Host	Mouse
Gene	PRKCA
Purity	Protein A purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Dilutions	ELISA Flow Cytometry Immunocytochemistry/ Immunofluorescence Immunohistochemistry Immunohistochemistry-Frozen Immunohistochemistry-Paraffin Immunoprecipitation Radioimmunoassay Western Blot
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Storage	Store at 4C in the dark.
Buffer	50mM Sodium Borate
Preservative	0.05% Sodium Azide
Purity	Protein A purified