We are looking into protein kinase C-alpha antibodies for immunohistochemistry on formalin fixed, paraffin embedded tissue. I see that you have many antibodies, including several monoclonals. I wanted to confirm with you that the following antibodies are specific for C-alpha. NB600-201=MC5 (an older paper suggested that this might react with alpha, beta, gamm), NB110-57356=Y124, NB110-57353=Y143. Also, if you have an comparator data or feedback regarding PKC-alpha antibodies for IHC on FFPE, that would be very helpful. We would like to pick 1 antibody that is easy to titer and robust, so that we can move forward with our studies.

Name: PKC alpha Antibody (G13-R)
Brand: Novus Biologicals
SKU: NBP1-30121
Price: 449 USD

Question

We are looking into protein kinase C-alpha antibodies for immunohistochemistry on formalin fixed, paraffin embedded tissue.  I see that you have many antibodies, including several monoclonals.  I wanted to confirm with you that the following antibodies are specific for C-alpha.  NB600-201=MC5 (an older paper suggested that this might react with alpha, beta, gamm), NB110-57356=Y124, NB110-57353=Y143.  Also, if you have an comparator data or feedback regarding PKC-alpha antibodies for IHC on FFPE, that would be very helpful. We would like to pick 1 antibody that is easy to titer and robust, so that we can move forward with our studies.

Accepted Answer

We have never heard any feedback that these cross-react with other PKC proteins. If you have seen data that MC5 may cross-react, then I would consider avoiding this antibody if you are concerned. The immunogens for NB110-57356 and NB110-57353 come from the N- and C-terminals, which is where the PKC proteins vary the most, so these are not expected to cross-react and we have had no feedback or testing data that suggests that they do. I would recommend NB110-57356, since it has been published with. You may want to review the published data to determine if it seems this antibody will suit your needs.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, RtSpecies Glossary
Applications	WB, ELISA, ICC/IF, IP
Clone	G13-R
Clonality	Monoclonal
Host	Rabbit
Conjugate	Unconjugated

Description	This antibody is immunoaffinity purified with immunogenic peptide as a ligand.
Additional Information	Recombinant Monoclonal Antibody.
Immunogen	Peptide derived from N-terminal sequence of human PKC alpha. Antibody recognizes the epitope located between Asn8 - Ala17.
Epitope	Asn8 - Ala17
Isotype	IgG
Clonality	Monoclonal
Host	Rabbit
Gene	PRKCA
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	20mM Tris/HCl (pH 8.0) and 10 mg/ml BSA
Preservative	0.05% Sodium Azide
Purity	Immunogen affinity purified

Gene Symbol	PRKCA
Entrez	5578(Human)
Uniprot	P17252()

PKC alpha Antibody (G13-R)

Species Glossary

Images

Product Details

Order Details

PKC alpha Antibody (G13-R) Summary

Applications/Dilutions

Packaging, Storage & Formulations

Notes

Alternate Names for PKC alpha Antibody (G13-R)

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for PKC alpha Antibody (NBP1-30121) (0)

Reviews for PKC alpha Antibody (NBP1-30121) (0)

Product General Protocols

Video Protocols

FAQs for PKC alpha Antibody (NBP1-30121). (Showing 1 - 1 of 1 FAQ).

Secondary Antibodies

Isotype Controls

Additional PKC alpha Products

Research Areas for PKC alpha Antibody (NBP1-30121)

Blogs on PKC alpha.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics