![Western Blot: Occludin Antibody (OCLN/2181) [NBP3-07137] - Western Blot Analysis of MCF-7 cell lysate with Occludin Mouse Monoclonal Antibody (OCLN/2181).](http://images.novusbio.com/fullsize/Occludin-Antibody-OCLN-2181-Western-Blot-NBP3-07137-img0003.jpg "Western Blot: Occludin Antibody (OCLN/2181) [NBP3-07137] - Western Blot Analysis of MCF-7 cell lysate with Occludin Mouse Monoclonal Antibody (OCLN/2181).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clone | OCLN/2181 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08879) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary) (Uniprot: Q16625) |
| Localization | Cell Surface and Cytoplasmic |
| Marker | Marker of Early Blood Brain Barrier Damage |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | OCLN |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Occludin Antibody (NBP3-07137)Find related products by research area.
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