Home » Neuropilin-2 » Neuropilin-2 Lysates » Neuropilin-2 Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

Neuropilin-2 Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: Neuropilin 2 Overexpression Lysate (Native) [NBP2-04605] - Neuropilin 2 Overexpression Lysate (Native) Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NRP2.
Western Blot: Neuropilin 2 Overexpression Lysate (Native) [NBP2-04605] - Neuropilin 2 Overexpression Lysate (Native) Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

100% Guarantee on every product

Neuropilin-2 Overexpression Lysate Summary

Description

Neuropilin-2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC210928

Accession#: NM_201267

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
NRP2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
98.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Neuropilin-2 Overexpression Lysate

  • MGC126574
  • neuropilin 2
  • Neuropilin2
  • Neuropilin-2
  • neuropilin-2a(22)
  • neuropilin-2b(0)
  • NP2
  • NPN 2
  • NPN2
  • PRO2714
  • receptor for VEGF165 and semaphorins class3
  • Vascular endothelial cell growth factor 165 receptor 2
  • vascular endothelial growth factor-165 receptor 2
  • VEGF1265R2
  • VEGF165R2neuropilin-2a(17)

Background

NRP2 encodes a member of the neuropilin family of receptor proteins. The encoded transmembrane protein binds to SEMA3C protein {sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C} and SEMA3F protein {sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F}, and interacts with vascular endothelial growth factor (VEGF). This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Multiple transcript variants encoding distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Neuropilin-1
Antibody [Unconjugate
AF566
Immunohistochemistry Neuropilin-1 Antibody [Unconjugated]
Immunohistochemistry Neuropilin-1 Antibody [Unconjugated]
Western Blot Neuropilin-1 Antibody [Unconjugated]
(10 Reviews)
(103 Publications)
Species: Mu, Rt
Applications: Block, CyTOF-ready, Flow, IHC, WB
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(672 Publications)
Species: Hu
Applications: ELISA
Semaphorin 3F
[Unconjugated]
3237-S3
Semaphorin 3F [Unconjugated]
(8 Publications)
Species: Mu
Applications: BA
NELL1 Antibody
AF7109
Western Blot NELL1 Antibody
Immunohistochemistry NELL1 Antibody
Species: Mu
Applications: IHC, WB
Semaphorin 3A
[Unconjugated]
1250-S3
Bioactivity Semaphorin 3A [Unconjugated]
(33 Publications)
Species: Hu
Applications: BA, BA
NAP1L1 Antibody
NBP1-81162
Immunohistochemistry-Paraffin NAP1L1 Antibody
Immunohistochemistry-Paraffin NAP1L1 Antibody
Immunohistochemistry-Paraffin NAP1L1 Antibody
(1 Publication)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
VEGFR2/KDR/Flk-1
Antibody [Unconjugate ...
AF644
Neutralization VEGFR2/KDR/Flk-1 Antibody [Unconjugated]
Immunohistochemistry VEGFR2/KDR/Flk-1 Antibody [Unconjugated]
Immunohistochemistry VEGFR2/KDR/Flk-1 Antibody [Unconjugated]
(3 Reviews)
(97 Publications)
Species: Mu
Applications: CyTOF-ready, Dual ISH-IHC, Flow, IHC, Neut, WB
Optineurin Antibody
NBP1-84682
Western Blot Optineurin Antibody
Western Blot Optineurin Antibody
Western Blot Optineurin Antibody
(2 Reviews)
(3 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
VEGFR1/Flt-1
Antibody [Unconjugate
AF321
Immunohistochemistry VEGFR1/Flt-1 Antibody [Unconjugated]
Immunohistochemistry VEGFR1/Flt-1 Antibody [Unconjugated]
Western Blot VEGFR1/Flt-1 Antibody [Unconjugated]
(2 Reviews)
(73 Publications)
Species: Hu
Applications: Block, CyTOF-ready, Flow, IHC, WB
Semaphorin 3B
Antibody
NB100-2218
Simple Western Semaphorin 3B Antibody
Western Blot Semaphorin 3B Antibody
Immunocytochemistry/ Immunofluorescence Semaphorin 3B Antibody
(2 Publications)
Species: Bv, Ca, Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
Neuronal Pentraxin
1
7707-NP
Neuronal Pentraxin 1
(1 Publication)
Species: Hu
Applications: BA
VEGF-C [HRP]
DVEC00
N/A VEGF-C [HRP]
N/A VEGF-C [HRP]
N/A VEGF-C [HRP]
(53 Publications)
Species: Hu
Applications: ELISA
Semaphorin 3F
[Unconjugated]
3237-S3
Semaphorin 3F [Unconjugated]
(8 Publications)
Species: Mu
Applications: BA
VEGFR3/Flt-4
Antibody [Unconjugate
AF743
Flow Cytometry VEGFR3/Flt-4 Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence VEGFR3/Flt-4 Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence VEGFR3/Flt-4 Antibody [Unconjugated]
(3 Reviews)
(136 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, WB
Semaphorin 6A
Antibody [Unconjugate
AF1615
Immunohistochemistry Semaphorin 6A Antibody [Unconjugated]
(7 Publications)
Species: Mu
Applications: IHC, WB
Plexin A3 Antibody
[Unconjugated]
AF4075
Immunohistochemistry Plexin A3 Antibody [Unconjugated]
Immunohistochemistry Plexin A3 Antibody [Unconjugated]
(4 Publications)
Species: Mu, Rt
Applications: IHC, WB

Publications for Neuropilin-2 Lysate (NBP2-04605) (0)

There are no publications for Neuropilin-2 Lysate (NBP2-04605).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Neuropilin-2 Lysate (NBP2-04605) (0)

There are no reviews for Neuropilin-2 Lysate (NBP2-04605). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Neuropilin-2 Lysate (NBP2-04605) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Neuropilin-2 Products

Research Areas for Neuropilin-2 Lysate (NBP2-04605)

Find related products by research area.

Blogs on Neuropilin-2

There are no specific blogs for Neuropilin-2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Neuropilin-2 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol NRP2