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MST2/STK3 Antibody (OTI4G10)

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Western Blot: MST2/STK3 Antibody (4G10) [NBP1-48017] - MST2 Antibody (4G10) [NBP1-48017] Analysis of extracts (35ug) from 9 different cell lines by using anti-MST2 monoclonal antibody.
Immunohistochemistry-Paraffin: MST2/STK3 Antibody (4G10) [NBP1-48017] - Staining of paraffin-embedded Ovary tissue using anti-MST2 mouse monoclonal antibody.
Western Blot: MST2/STK3 Antibody (4G10) [NBP1-48017] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MST2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunohistochemistry-Paraffin: MST2/STK3 Antibody (4G10) [NBP1-48017] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-MST2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MST2/STK3 Antibody (4G10) [NBP1-48017] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-MST2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MST2/STK3 Antibody (4G10) [NBP1-48017] - Staining of paraffin-embedded colon tissue using anti-MST2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MST2/STK3 Antibody (4G10) [NBP1-48017] - Staining of paraffin-embedded endometrium tissue using anti-MST2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MST2/STK3 Antibody (4G10) [NBP1-48017] - Staining of paraffin-embedded Kidney tissue using anti-MST2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MST2/STK3 Antibody (4G10) [NBP1-48017] - Staining of paraffin-embedded liver tissue using anti-MST2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MST2/STK3 Antibody (4G10) [NBP1-48017] - Staining of paraffin-embedded lung tissue using anti-MST2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, IHC
Clone
OTI4G10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

MST2/STK3 Antibody (OTI4G10) Summary

Immunogen
Full-length protein expressed in 293T cell transfected with human STK3 expression vector
Specificity
This antibody is specific for Homo sapiens serine/threonine kinase 3 (STE20 homolog, yeast) (STK3).
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
STK3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
Theoretical MW
56.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MST2/STK3 Antibody (OTI4G10)

  • EC 2.7.11
  • FLJ90748
  • KRS1
  • Mammalian STE20-like protein kinase 2
  • Mess1
  • MST2
  • MST-2
  • MST2EC 2.7.11.1
  • MST3
  • serine/threonine kinase 3 (STE20 homolog, yeast)
  • serine/threonine kinase 3 (Ste20, yeast homolog)
  • serine/threonine kinase 3
  • serine/threonine-protein kinase 3
  • Serine/threonine-protein kinase Krs-1
  • STE20-like kinase MST2
  • STK3

Background

Protein kinase activation is a frequent response of cells to treatment with growth factors,chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast ' sterile 20'(Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for MST2/STK3 Antibody (NBP1-48017). (Showing 1 - 1 of 1 FAQ).

  1. Do you have any information on how this antibody was generated?
    • The immunogen used to generate this antibody is the full-length protein. Unfortunately, we have not epitope mapped this clone; however, given that it is a licensed clone, there may be some publication information available with further details of the epitope.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STK3
Entrez