I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?

Name: Mouse/Rat FGF basic/FGF2/bFGF Quantikine ELISA Kit
Brand: Novus Biologicals
SKU: MFB00
Price: 635 USD

Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have several antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

Mouse/Rat FGF basic/FGF2/bFGF Quantikine ELISA Kit (MFB00): Novus Biologicals

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Mu, RtSpecies Glossary
Applications	ELISA
Conjugate	HRP

Background	The Quantikine Mouse/Rat FGF basic Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat FGF basic in cell culture supernates, tissue lysates, serum, and plasma. It contains E. coli-expressed recombinant rat FGF basic and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. ...Results obtained using natural FGF basic showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural FGF basic. Show More
Specificity	Natural and recombinant FGF basic. This assay cross-reacts 100% with Bovine FGF basic, recombinant Human FGF basic is detectable.
Assay Type	Solid Phase Sandwich ELISA
Inter-Assay	See PDF Datasheet for details
Intra-Assay	See PDF Datasheet for details
Spike Recovery	See PDF Datasheet for details
Sample Volume	See PDF Datasheet for details
Gene	Fgf2

Publications using MFB00	Applications	Species
Wu, Y;Sun, J;Lin, Q;Wang, D;Hai, J; Sustained release of vascular endothelial growth factor A and basic fibroblast growth factor from nanofiber membranes reduces oxygen/glucose deprivation-induced injury to neurovascular units Neural regeneration research 2024-04-01 [PMID: 37843225]
H Ben Amara, DC Martinez, FA Shah, AJ Loo, L Emanuelsso, B Norlindh, R Willumeit-, T Plocinski, W Swieszkows, A Palmquist, O Omar, P Thomsen Magnesium implant degradation provides immunomodulatory and proangiogenic effects and attenuates peri-implant fibrosis in soft tissues Bioactive materials, 2023-03-16;26(0):353-369. 2023-03-16 [PMID: 36942009] (Rat)		Rat
W Ji, Z Wu, J Wen, H Tang, Z Chen, B Xue, Z Tian, Y Ba, N Zhang, X Wen, B Hou A simple method to isolate structurally and chemically intact brain vascular basement membrane for neural regeneration following traumatic brain injury Biomaterials research, 2023-01-12;27(1):2. 2023-01-12 [PMID: 36635718] (MFB00)		MFB00
Y Matsuno, M Yanagihara, K Ueno, T Saito, H Kurazumi, R Suzuki, S Katsura, A Oga, K Hamano Dry preserved multilayered fibroblast cell sheets are a new manageable tool for regenerative medicine to promote wound healing Scientific Reports, 2022-07-22;12(1):12519. 2022-07-22 [PMID: 35869108] (Mouse)		Mouse
M Furuhata, T Takayama, T Yamamoto, Y Ozawa, M Senoo, M Ozaki, S Yamano, S Sato Real-time assessment of guided bone regeneration in critical size mandibular bone defects in rats using collagen membranes with adjunct fibroblast growth factor-2 Journal of dental sciences, 2021-04-03;16(4):1170-1181. 2021-04-03 [PMID: 34484585] (Rat)		Rat
H Nagano, Y Suematsu, M Takuma, S Aoki, A Satoh, E Takayama, M Kinoshita, Y Morimoto, S Takeoka, T Fujie, T Kiyosawa Enhanced cellular engraftment of adipose-derived mesenchymal stem cell spheroids by using nanosheets as scaffolds Scientific Reports, 2021-07-14;11(1):14500. 2021-07-14 [PMID: 34262089] (Mouse)		Mouse
T Murakami, D Matsugami, W Yoshida, K Imamura, T Bizenjima, F Seshima, A Saito Healing of Experimental Periodontal Defects Following Treatment with Fibroblast Growth Factor-2 and Deproteinized Bovine Bone Mineral Biomolecules, 2021-05-29;11(6):. 2021-05-29 [PMID: 34072351] (Rat)		Rat
HY Kim, SY Chun, EH Lee, B Kim, YS Ha, JW Chung, JN Lee, BS Kim, SH Oh, TG Kwon Bladder Regeneration Using a Polycaprolactone Scaffold with a Gradient Structure and Growth Factors in a Partially Cystectomized Rat Model J Korean Med Sci, 2020-10-26;35(41):e374. 2020-10-26 [PMID: 33107231] (Rat)		Rat
R Zilkha-Fal, T Rachutin-Z, L Cleaver, M Gurevich, A Achiron RAM-589.555 favors neuroprotective and anti-inflammatory profile of CNS-resident glial cells in acute relapse EAE affected mice J Neuroinflammation, 2020-10-21;17(1):313. 2020-10-21 [PMID: 33081798] (Mouse)		Mouse
YJ Park, HT An, JS Park, O Park, AJ Duh, K Kim, KH Chung, KC Lee, Y Oh, S Lee Tyrosine kinase inhibitor neratinib attenuates liver fibrosis by targeting activated hepatic stellate cells Sci Rep, 2020-09-08;10(1):14756. 2020-09-08 [PMID: 32901093] (Mouse)		Mouse
N Firoozi, Y Kang Immobilization of FGF on Poly(xylitol dodecanedioic Acid) Polymer for Tissue Regeneration Sci Rep, 2020-06-26;10(1):10419. 2020-06-26 [PMID: 32591607] (Mouse)		Mouse
Z Sun, Y Xie, RJ Lee, Y Chen, Q Jin, Q Lv, J Wang, Y Yang, Y Li, Y Cai, R Wang, Z Han, L Zhang, M Xie Myocardium-targeted transplantation of PHD2 shRNA-modified bone mesenchymal stem cells through ultrasound-targeted microbubble destruction protects the heart from acute myocardial infarction Theranostics, 2020-04-06;10(11):4967-4982. 2020-04-06 [PMID: 32308762] (Rat)		Rat
A Shima, A Itou, S Takeuchi Cell fibers promote proliferation of co-cultured cells on a dish Sci Rep, 2020-01-14;10(1):288. 2020-01-14 [PMID: 31937888] (Mouse)		Mouse
Z Zhang, R Qu, T Fan, J Ouyang, F Lu, J Dai Stepwise Adipogenesis of Decellularized Cellular Extracellular Matrix Regulates Adipose Tissue-Derived Stem Cell Migration and Differentiation Stem Cells Int, 2019-11-06;2019(0):1845926. 2019-11-06 [PMID: 31781233] (Rat)		Rat
C Li, J Pan, L Ye, H Xu, B Wang, H Xu, L Xu, T Hou, D Zhang Autophagy regulates the therapeutic potential of adipose-derived stem cells in LPS-induced pulmonary microvascular barrier damage Cell Death Dis, 2019-10-23;10(11):804. 2019-10-23 [PMID: 31645547] (Mouse)		Mouse
YZ Zhou, Z Cheng, Y Wu, QY Wu, XB Liao, Y Zhao, JM Li, XM Zhou, XM Fu Mesenchymal stem cell-derived conditioned medium attenuate angiotensin II-induced aortic aneurysm growth by modulating macrophage polarization J. Cell. Mol. Med., 2019-10-04;0(0):. 2019-10-04 [PMID: 31583844] (Mouse)		Mouse
R Fu, CF Han, T Ni, L Di, LJ Liu, WC Lv, YR Bi, N Jiang, Y He, HM Li, S Wang, H Xie, BA Chen, XS Wang, SJ Weiss, T Lu, QL Guo, ZQ Wu A ZEB1/p53 signaling axis in stromal fibroblasts promotes mammary epithelial tumours Nat Commun, 2019-07-19;10(1):3210. 2019-07-19 [PMID: 31324807] (Mouse)		Mouse
MN Lee, HS Hwang, SH Oh, A Roshanzade, JW Kim, JH Song, ES Kim, JT Koh Elevated extracellular calcium ions promote proliferation and migration of mesenchymal stem cells via increasing osteopontin expression Exp. Mol. Med., 2018-11-05;50(11):142. 2018-11-05 [PMID: 30393382] (Mouse)		Mouse
D Li, P Cheng, H Jiang, T Cao, J Wang, Y Gao, Y Lin, C Wang, S Zhang, J Li, B Liu, Y Song, L Yang, G Pei Vascularization converts the lineage fate of bone mesenchymal stem cells to endothelial cells in tissue-engineered bone grafts by modulating FGF2-RhoA/ROCK signaling Cell Death Dis, 2018-09-20;9(10):959. 2018-09-20 [PMID: 30237398] (Rat)		Rat
C Chen, H Li, J Jiang, Q Zhang, F Yan Inhibiting PHD2 in bone marrow mesenchymal stem cells via lentiviral vector-mediated RNA interference facilitates the repair of periodontal tissue defects in SD rats Oncotarget, 2017-08-14;8(42):72676-72699. 2017-08-14 [PMID: 29069818] (Rat)		Rat
T Shimizu, N Narang, P Chen, B Yu, M Knapp, J Janardanan, J Blair, JK Liao Fibroblast deletion of ROCK2 attenuates cardiac hypertrophy, fibrosis, and diastolic dysfunction JCI Insight, 2017-07-06;2(13):. 2017-07-06 [PMID: 28679962] (Rat)		Rat
Disrupting the blood-brain barrier by focused ultrasound induces sterile inflammation Proc. Natl. Acad. Sci. U.S.A, 2016-12-19;0(0):. 2016-12-19 [PMID: 27994152] (Rat)		Rat
Shimizu S, Shimizu T, Tsounapi P, Higashi Y, Martin D, Nakamura K, Honda M, Inoue K, Saito M Effect of Silodosin, an Alpha1A-Adrenoceptor Antagonist, on Ventral Prostatic Hyperplasia in the Spontaneously Hypertensive Rat. PLoS ONE, 2015-08-26;10(8):e0133798. 2015-08-26 [PMID: 26308715] (Rat)		Rat
Toyoshima A, Yasuhara T, Kameda M, Morimoto J, Takeuchi H, Wang F, Sasaki T, Sasada S, Shinko A, Wakamori T, Okazaki M, Kondo A, Agari T, Borlongan C, Date I Intra-Arterial Transplantation of Allogeneic Mesenchymal Stem Cells Mounts Neuroprotective Effects in a Transient Ischemic Stroke Model in Rats: Analyses of Therapeutic Time Window and Its Mechanisms. PLoS ONE, 2015-06-15;10(6):e0127302. 2015-06-15 [PMID: 26075717] (Rat)		Rat
Mellott T, Pender S, Burke R, Langley E, Blusztajn J IGF2 ameliorates amyloidosis, increases cholinergic marker expression and raises BMP9 and neurotrophin levels in the hippocampus of the APPswePS1dE9 Alzheimer's disease model mice. PLoS ONE, 2014-04-14;9(4):e94287. 2014-04-14 [PMID: 24732467] (Mouse)		Mouse
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Mouse/Rat FGF basic/FGF2/bFGF Quantikine ELISA Kit

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