Mouse Mesenchymal Stem Cell Flow Cytometry Kit Summary
Description |
This product is designed for the flow cytometric analysis of mouse MSCs using four fluorochrome-conjugated antibodies. Features- Verifies MSC identity in less than 2 hours
- Conjugated antibodies allow for single step staining
- Defines the starting cell population
- Multiple markers reduce experimental variation
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Kit Type |
Flow Cytometry Kit |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Store at 4C in the dark. |
Kit Components
Components
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- 100 mL of 1X Staining Buffer
- 250 uL of Rat IgG2A-APC Isotype Control
- 250 uL of Rat IgG2A-CFS Isotype Control
- 250 uL of Rat IgG2A-PE Isotype Control
- 250 uL of Rat IgG2B-PerCP Isotype Control
- Negative Marker: 250 uL of CD45-PerCP Rat IgG2B; Clone 30-F11
- Positive Marker: 250 uL of CD105/Endoglin-CFS Rat IgG2A; Clone 209701
- Positive Marker: 250 uL of CD29/Integrin beta1-PE Rat IgG2A; Clone 265917
- Positive Marker: 250 uL of Sca-1-APC Rat IgG2A; Clone 177228
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Background
The term "mesenchymal stem cells" (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. However, because these biological properties and hierarchical relationships remain to be clearly demonstrated in vivo, the term "multipotent mesenchymal stromal cells" is often used to distinguish cultured cells from their in vivo precursors. Originally discovered in mouse bone marrow, multipotent mesenchymal stromal cells cultured from a variety of species and tissue types, have been shown to differentiate into progeny of additional lineages including, cardiomyocytes, endothelial cells, hepatocytes, and neural cells. Again, the physiological relevance of these findings remains to be determined.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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