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Moesin Antibody (rMSN/492) [Alexa Fluor® 350]

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Moesin Antibody (rMSN/492) [Alexa Fluor® 350] [NBP3-08923AF350] - Vial of Alexa Fluor 350 conjugated antibody. Alexa Fluor 350 is optimally excited at 346 nm by the UV laser (350 or 355 nm) and has an emission maximum of 442 nm.
Moesin Antibody (rMSN/492) [Alexa Fluor® 350] [NBP3-08923AF350] - Vial of Alexa Fluor 350 conjugated antibody. Alexa Fluor 350 is optimally excited at 346 nm by the UV laser (350 or 355 nm) and has an emission ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
rMSN/492
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 350

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP3-08923AF405
Alexa Fluor 488
NBP3-08923AF488
Alexa Fluor 532
NBP3-08923AF532
Alexa Fluor 594
NBP3-08923AF594
Alexa Fluor 647
NBP3-08923AF647
Alexa Fluor 700
NBP3-08923AF700
Alexa Fluor 750
NBP3-08923AF750
Allophycocyanin
NBP3-08923APC
Biotin
NBP3-08923B
CoraFluor 1
NBP3-08923CL1
DyLight 350
NBP3-08923UV
DyLight 405
NBP3-08923V
DyLight 488
NBP3-08923G
DyLight 550
NBP3-08923R
DyLight 594
NBP3-08923DL594
DyLight 650
NBP3-08923C
DyLight 680
NBP3-08923FR
DyLight 755
NBP3-08923IR
FITC
NBP3-08923F
HRP
NBP3-08923H
Janelia Fluor 525
NBP3-08923JF525
Janelia Fluor 549
NBP3-08923JF549
Janelia Fluor 585
NBP3-08923JF585
Janelia Fluor 635
NBP3-08923JF635
Janelia Fluor 646
NBP3-08923JF646
Janelia Fluor 669
NBP3-08923JF669
PE
NBP3-08923PE
PerCP
NBP3-08923PCP
Unconjugated
NBP3-07218-100ug
mFluor Violet 450 SE
NBP3-08923MFV450
mFluor Violet 500 SE
NBP3-08923MFV500
mFluor Violet 610 SE
NBP3-08923MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-07218-100ug
Azide and BSA Free
NBP3-08923
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Moesin Antibody (rMSN/492) [Alexa Fluor® 350] Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant full-length human Moesin protein (Uniprot: P26038)
Localization
Cytoplasmic & Cell Surface
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MSN
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Moesin Antibody (rMSN/492) [Alexa Fluor® 350]

  • Membrane-organizing extension spike protein
  • moesin

Background

Moesin (membrane-organizing extension spike protein) has previously been characterized as a possible receptor protein for heparan sulfate and also as a cytoskeletal linker protein that stabilizes cell surface microvilli, filopodia and lamellipodia. Data indicate that moesin is identical to the 77-kDa band that copurifies with ezrin in its isolation from human placenta (1). Members of the ezrin-radixin-moesin (ERM) family of membrane-cytoskeletal linking proteins have NH2- and COOH-terminal domains that associate with the plasma membrane and the actin cytoskeleton, respectively (2). It has been demonstrated that ezrin-radixin-moesin proteins are rapidly inactivated after antigen recognition through a Vav1-Rac1 pathway. The resulting disanchoring of the cortical actin cytoskeleton from the plasma membrane decreased cellular rigidity, leading to more efficient T cell-antigen-presenting cell conjugate formation (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Moesin Antibody (NBP3-08923AF350). (Showing 1 - 1 of 1 FAQ).

  1. I am looking to use shRNA to inhibit Moesin expression. I have had people advise me that my initial MOI should be low as 'less is more' and 'a little goes a long way' in terms of siRNA. I was wondering if you could elaborate on this for me and explain why my initial MOI should be low.
    • The reason for a low MOI is most likely because RNAi is a very strong and efficient technique. Wikipedia does a good job of explaining <a href="http://en.wikipedia.org/wiki/RNA_interference" target="_blank">RNA interference</a>. However, I would imagine that in a cell, there will be at most 1-2 copies of the gene mRNA present at any given time, unless you're dealing with a highly expressed protein such as Actin, where I would imagine silencing Actin would be lethal to the cell. I can imagine a few reasons to not use too much siRNA. First, it is expensive, so you don't want to waste it. Second, using too much would cause there to be a lot of non-translatable RNA present in the cell, which could trigger an immune response, as the presence of uncapped RNAs can indicate presence of a virus and one of the TLRs may respond to this.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MSN