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MMP-10 Antibody

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Western Blot: MMP-10 Antibody [NBP2-17309] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MMP10 antibody [N1C1] diluted at 1:1000.
Immunohistochemistry-Paraffin: MMP10 Antibody [NBP2-17309] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.
Western Blot: MMP-10 Antibody [NBP2-17309] - Non-transfected (-) and MMP10-transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP10 antibody diluted at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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MMP-10 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human MMP-10. The exact sequence is proprietary.
Localization
Secreted; extracellular space; extracellular matrix
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MMP10
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (82%), Rat (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for MMP-10 Antibody

  • EC 3.4.24
  • EC 3.4.24.22
  • matrix metallopeptidase 10 (stromelysin 2)
  • matrix metalloprotease 10
  • matrix metalloproteinase 10 (stromelysin 2)
  • Matrix metalloproteinase-10
  • MMP10
  • MMP-10
  • SL-2
  • STMY2stromelysin-2
  • Stromelysin 2
  • transin 2
  • transin-2

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans and fibronectin. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MMP10
Uniprot