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MGMT Antibody (MGMT/4791)

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Monospecific Mouse Monoclonal Antibody to MGMT (MGMT/4791).
Analysis of Protein Array containing more than 19,000 full-length human proteins using Monospecific Mouse Monoclonal Antibody to MGMT (MGMT/4791).
Formalin-fixed, paraffin-embedded human lymph node stained with MGMT antibody (MGMT/4791).
Formalin-fixed, paraffin-embedded human glioma stained with MGMT antibody (MGMT/4791).
Formalin-fixed, paraffin-embedded human testis stained with MGMT antibody (MGMT/4791).
Formalin-fixed, paraffin-embedded human stomach stained with MGMT antibody (MGMT/4791).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
MGMT/4791
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-21103AF350
Alexa Fluor 405
NBP3-21103AF405
Alexa Fluor 488
NBP3-21103AF488
Alexa Fluor 532
NBP3-21103AF532
Alexa Fluor 594
NBP3-21103AF594
Alexa Fluor 647
NBP3-21103AF647
Alexa Fluor 700
NBP3-21103AF700
Alexa Fluor 750
NBP3-21103AF750
Allophycocyanin
NBP3-21103APC
Biotin
NBP3-21103B
CoraFluor 1
NBP3-21103CL1
DyLight 350
NBP3-21103UV
DyLight 405
NBP3-21103V
DyLight 488
NBP3-21103G
DyLight 550
NBP3-21103R
DyLight 594
NBP3-21103DL594
DyLight 650
NBP3-21103C
DyLight 680
NBP3-21103FR
DyLight 755
NBP3-21103IR
FITC
NBP3-21103F
HRP
NBP3-21103H
Janelia Fluor 525
NBP3-21103JF525
Janelia Fluor 549
NBP3-21103JF549
Janelia Fluor 585
NBP3-21103JF585
Janelia Fluor 635
NBP3-21103JF635
Janelia Fluor 646
NBP3-21103JF646
Janelia Fluor 669
NBP3-21103JF669
PE
NBP3-21103PE
PerCP
NBP3-21103PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP3-21103
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MGMT Antibody (MGMT/4791) Summary

Description
Positive Controls: Jurkat or MCF-7 cells. Human tonsil or stomach (IHC).

Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Non-hazardous. No MSDS required.
Immunogen
Purified His-tagged MGMT protein
Localization
Nucleus
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MGMT
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for MGMT Antibody (MGMT/4791)

  • 6-O-methylguanine-DNA methyltransferase
  • EC 2.1.1.63
  • methylated-DNA--protein-cysteine methyltransferase
  • methylguanine-DNA methyltransferase
  • MGMT
  • O-6-methylguanine-DNA methyltransferase
  • O6-methylguanine-DNA methyltransferase
  • O-6-methylguanine-DNA-alkyltransferase

Background

O6-Methylguanine-DNA Methyltransferase (MGMT) is an important DNA repair protein involved in tumor cell resistance to the cytotoxic activity of chemotherapeutic alkylating agents. This protein is also effective in protecting normal cells against the genotoxic and carcinogenic effects of DNA alkylation. The alkylating drug resistance is caused by MGMT's ability to remove DNA alkyl groups introduced in the O6 position of guanine. MGMT is expressed in highly variable amounts, depending upon the cell and tissue type, species, and cellular growth characteristics. In addition, MGMT activity varies among groups of tumors and within a particular type of tumor.

MGMT antibodes are useful tools for DNA repair studies and for some cancer research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MGMT