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MEF2A Antibody (1A10)

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Western Blot: MEF2A Antibody (1A10) [H00004205-M02] - MEF2A monoclonal antibody (M02), clone 1A10. Analysis of MEF2A expression in human lung cancer.
Immunocytochemistry/ Immunofluorescence: MEF2A Antibody (1A10) [H00004205-M02] - Analysis of monoclonal antibody to MEF2A on HeLa cell . Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: MEF2A Antibody (1A10) [H00004205-M02] - Analysis of monoclonal antibody to MEF2A on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.
Sandwich ELISA: MEF2A Antibody (1A10) [H00004205-M02] - Detection limit for recombinant GST tagged MEF2A is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
1A10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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MEF2A Antibody (1A10) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
MEF2A (AAH13437, 71 a.a. ~ 170 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EYNEPHESRTNSDIVEALNKKEHRGCDSPDPDTSYVLTPHTEEKYKKINEEFDNMMRNHKIAPGLPPQNFSMSVTVPVTSPNALSYTNPGSSLVSPSLAA
Specificity
MEF2A (1A10)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MEF2A
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:500
Application Notes
Antibody reactivity against tissue lysate and recombinant protein for WB. It has been used for IHC-P and ELISA.
Control
Human Lung Whole Tissue Lysate (Adult Whole Tumor)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MEF2A Antibody (1A10)

  • ADCAD1
  • MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancerfactor 2A)
  • MEF2
  • myocyte enhancer factor 2A
  • myocyte-specific enhancer factor 2A
  • RSRFC4
  • RSRFC9
  • Serum response factor-like protein 1

Background

The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including myoD (MIM 159970), myogenin (MIM 159980), MYF5 (MIM 159990), and MRF4 (MIM 159991) are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. The MEF2 genes are members of the MADS gene family (named for the yeast mating type-specific transcription factor MCM1, the plant homeotic genes 'agamous' and 'deficiens' and the human serum response factor SRF (MIM 600589)), a family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MEF2A Antibody (H00004205-M02) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MEF2A
Entrez
OMIM
Uniprot