![Western Blot: MeCP2 Antibody [NBP2-50060] - Analysis of tissue and cell lysates using rabbit pAb to MeCP2, NBP2-50060, dilution 1:20,000 in green: [1] protein standard (red), [2] Rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain, [6] HeLa cells, [7] C6 cells. Major band at about 70-75 kDa corresponds to MeCP2 protein, predominantly detected in the nuclear fraction of the lysates. The apparent molecular weights of MeCP2 based on SDS-PAGE mobility are variable between species and higher than the actual MeCP2 molecular weight which is ~54kDa](http://images.novusbio.com/fullsize/MeCP2-Antibody-Western-Blot-NBP2-50060-img0005.jpg "Western Blot: MeCP2 Antibody [NBP2-50060] - Analysis of tissue and cell lysates using rabbit pAb to MeCP2, NBP2-50060, dilution 1:20,000 in green: [1] protein standard (red), [2] Rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain, [6] HeLa cells, [7] C6 cells. Major band at about 70-75 kDa corresponds to MeCP2 protein, predominantly detected in the nuclear fraction of the lysates. The apparent molecular weights of MeCP2 based on SDS-PAGE mobility are variable between species and higher than the actual MeCP2 molecular weight which is ~54kDa")
| Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length recombinant human MeCP2 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MECP2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In western blot, the apparent molecular weights of MeCP2 based on SDS-PAGE mobility are variable between species. |
| Theoretical MW | 75 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 50% glycerol |
| Preservative | 0.035% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for MeCP2 Antibody (NBP2-50060)Find related products by research area.
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New MECP2 Study Suggests Important Link to GABA MECP2 antibodies are used in DNA methylation studies as well as research into Rett syndrome, a progressive neurological disorder caused by a mutation in the MECP2 gene. Now, a new study has been published by the laboratory where the Rett Syndrome gene... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NBP2-50060] - Mouse brain section, which was cut at 45um on a vibratome after perfusion with 4% paraformaldehyde and overnight fixation, was stained with rabbit polyclonal antibody NBP2-50060. MeCP2 was mainly associated with methylated DNA in nucleus of neuron cells (green). Blue shows DAPI staining of nuclear DNA.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-50060-img0003.jpg "Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NBP2-50060] - Mouse brain section, which was cut at 45um on a vibratome after perfusion with 4% paraformaldehyde and overnight fixation, was stained with rabbit polyclonal antibody NBP2-50060. MeCP2 was mainly associated with methylated DNA in nucleus of neuron cells (green). Blue shows DAPI staining of nuclear DNA.")
![Immunohistochemistry Free-Floating: MeCP2 Antibody [NBP2-50060] - Analysis of rat cerebellum section costained with rabbit pAb to MeCP2, NBP2-50060, dilution 1:5,000, in red, and chicken pAb to calbindin, dilution 1:2,000, in green. Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with above antibodies. The MeCP2 antibody selectively stains nuclei of neuronal cells. Calbindin, often used as Purkinje cell marker, is prominently expressed in dendrites and perikarya of these cells in the cerebellar molecular layer.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-50060-img0004.jpg "Immunohistochemistry Free-Floating: MeCP2 Antibody [NBP2-50060] - Analysis of rat cerebellum section costained with rabbit pAb to MeCP2, NBP2-50060, dilution 1:5,000, in red, and chicken pAb to calbindin, dilution 1:2,000, in green. Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with above antibodies. The MeCP2 antibody selectively stains nuclei of neuronal cells. Calbindin, often used as Purkinje cell marker, is prominently expressed in dendrites and perikarya of these cells in the cerebellar molecular layer.")
![Western Blot: MeCP2 Antibody [NBP2-50060] - Analysis of MeCP2 expression in nuclear extracts from mouse brain with NBP2-50060. This polyclonal antibody recognizes a strong and clear band at 75 kDa corresponding to total MeCP2 of mouse brain in SDS-PAGE, while molecular weight of MeCP2 protein is 54 kDa.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Western-Blot-NBP2-50060-img0002.jpg "Western Blot: MeCP2 Antibody [NBP2-50060] - Analysis of MeCP2 expression in nuclear extracts from mouse brain with NBP2-50060. This polyclonal antibody recognizes a strong and clear band at 75 kDa corresponding to total MeCP2 of mouse brain in SDS-PAGE, while molecular weight of MeCP2 protein is 54 kDa.")
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