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MCAM/CD146 Antibody (404722) [Phycoerythrin]

 (3 Publications)
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PC-12 rat adrenal pheochromocytoma cell line was stained with Mouse Anti-Rat MCAM/CD146 PE-conjugated Monoclonal Antibody (Catalog # FAB3250P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.
PC-12 rat adrenal pheochromocytoma cell line was stained with Mouse Anti-Rat MCAM/CD146 PE-conjugated Monoclonal Antibody (Catalog # FAB3250P, filled histogram) or isotype control antibody (Catalog # IC003P, open ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow
Clone
404722
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB3250U-100UG
Alexa Fluor 405
FAB3250V-100UG
Alexa Fluor 488
FAB3250G-100UG
Alexa Fluor 532
FAB3250X
Alexa Fluor 594
FAB3250T-100UG
Alexa Fluor 647
FAB3250R-100UG
Alexa Fluor 700
FAB3250N-100UG
Alexa Fluor 750
FAB3250S-100UG
Allophycocyanin
FAB3250A-100UG
Allophycocyanin/Cy7
FAB3250APCCY7
Biotin
FAB3250B
CoraFluor 1
FAB3250CL1
DyLight 350
FAB3250D
DyLight 405
FAB3250E
DyLight 488
FAB3250K
DyLight 550
FAB3250L
DyLight 594
FAB3250M
DyLight 650
FAB3250W
DyLight 680
FAB3250Y
DyLight 755
FAB3250Z
HRP
FAB3250H
Janelia Fluor 525
FAB3250JF525
Janelia Fluor 549
FAB3250I
Janelia Fluor 585
FAB3250JF585
Janelia Fluor 635
FAB3250JF635
Janelia Fluor 646
FAB3250J
Janelia Fluor 669
FAB3250JF669
PE/Atto594
FAB3250PEATT594
PE/Cy5.5
FAB3250PECY55
PE/Cy7
FAB3250PECY7
PerCP
FAB3250C
Unconjugated
MAB3250
mFluor Violet 450 SE
FAB3250MFV450
mFluor Violet 500 SE
FAB3250MFV500
mFluor Violet 610 SE
FAB3250MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB3250
Carrier Free
MAB3250
Carrier Free
MAB3250-SP
Carrier Free
FAB3250V-100UG
Carrier Free
FAB3250U-100UG
Carrier Free
FAB3250T-100UG
Carrier Free
FAB3250S-100UG
Carrier Free
FAB3250R-100UG
Carrier Free
FAB3250N-100UG
Carrier Free
FAB3250G-100UG
Carrier Free
FAB3250A-100UG
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MCAM/CD146 Antibody (404722) [Phycoerythrin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant rat MCAM/CD146
Arg19-Lys560
Accession # Q9EPF2
Specificity
Detects rat MCAM/CD146 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) MCAM, rhBCAM, or rhALCAM is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MCAM
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Publications
Read Publications using
FAB3250P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MCAM/CD146 Antibody (404722) [Phycoerythrin]

  • CD146 antigen
  • CD146
  • cell surface glycoprotein MUC18
  • Cell surface glycoprotein P1H12
  • L-Gicerin
  • MCAM
  • melanoma adhesion molecule
  • melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen
  • Melanoma-associated antigen A32
  • Melanoma-associated antigen MUC18
  • MUC18
  • MUC18Gicerin

Background

Melanoma Cell Adhesion Molecule (MCAM), also known as CD146 and MUC18, is an Ig-superfamily adhesion molecule that is expressed on endothelial cells, neurons and a variety of tumor cells. MCAM is associated with tumor progression and metastasis and may be involved in embryonic neural development. An alternately spliced isoform of rat MCAM has a 40 residue deletion in the cytoplasmic domain. Within the extracellular region, rat MCAM shares 72% and 90% amino acid sequence identity with human and mouse MCAM, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MCAM/CD146 Antibody (FAB3250P)(3)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: Flow Cytometry.


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Bioinformatics

Gene Symbol MCAM
Uniprot