Home » Lymphotoxin-alpha/TNF-beta » Lymphotoxin-alpha/TNF-beta Antibodies » Lymphotoxin-alpha/TNF-beta Antibody (3F12.2D3) [CoraFluor™ 1]
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Lymphotoxin-alpha/TNF-beta Antibody (3F12.2D3) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, B/N, SPR
Clone
3F12.2D3
Clonality
Monoclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-81105AF350
Alexa Fluor 405
NBP2-81105AF405
Alexa Fluor 488
NBP2-81105AF488
Alexa Fluor 532
NBP2-81105AF532
Alexa Fluor 594
NBP2-81105AF594
Alexa Fluor 647
NBP2-81105AF647
Alexa Fluor 700
NBP2-81105AF700
Alexa Fluor 750
NBP2-81105AF750
Allophycocyanin
NBP2-81105APC
Biotin
NBP2-81105B
DyLight 350
NBP2-81105UV
DyLight 405
NBP2-81105V
DyLight 488
NBP2-81105G
DyLight 550
NBP2-81105R
DyLight 594
NBP2-81105DL594
DyLight 650
NBP2-81105C
DyLight 680
NBP2-81105FR
DyLight 755
NBP2-81105IR
FITC
NBP2-81105F
HRP
NBP2-81105H
Janelia Fluor 525
NBP2-81105JF525
Janelia Fluor 549
NBP2-81105JF549
Janelia Fluor 585
NBP2-81105JF585
Janelia Fluor 635
NBP2-81105JF635
Janelia Fluor 646
NBP2-81105JF646
Janelia Fluor 669
NBP2-81105JF669
PE
NBP2-81105PE
PerCP
NBP2-81105PCP
Unconjugated
NBP2-81105-0.2mg
mFluor Violet 450 SE
NBP2-81105MFV450
mFluor Violet 500 SE
NBP2-81105MFV500
mFluor Violet 610 SE
NBP2-81105MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-81105-0.2mg
Azide and BSA Free
NBP2-81105-0.2mg
Azide and BSA Free
NBP2-81105-0.025mg
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Lymphotoxin-alpha/TNF-beta Antibody (3F12.2D3) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTalpha expressed in E. coli - the hybridoma clone was selected for its ability to bind LTalpha3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.
Specificity
3F12 binds to human LTalpha3 and LT-alpha-beta (with a KD of ~0.3 nM, determined by BIACORE; ~ 37 pM, determined by ELISA) in both its soluble homotrimeric and membrane heterotrimeric forms. LTalpha belongs to the tumor necrosis factor (TNF) superfamily and is secreted as a homo-trimer (LTalpha3), or is expressed on the cell surface in complex with LTbeta. Lymphotoxin is produced by lymphocytes and in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. LTalpha3 signaling induces target cells to upregulate many chemokines and cytokines in an NFkB-dependent manner.
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
LTA
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Neutralization
  • Surface Plasmon Resonance
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Lymphotoxin-alpha/TNF-beta Antibody (3F12.2D3) [CoraFluor™ 1]

  • LT
  • LTA
  • LT-alpha
  • lymphotoxin alpha (TNF superfamily, member 1)
  • Lymphotoxin alpha
  • Lymphotoxinalpha
  • Lymphotoxin-alpha
  • tnfb
  • TNF-beta
  • TNFBlymphotoxin-alpha
  • TNFSF1
  • TNFSF1B
  • TNFSF1TNF-beta
  • tumor necrosis factor beta
  • Tumor necrosis factor ligand superfamily member 1

Background

TNF-beta (tumor necrosis factor beta, lymphotoxin alpha, TNFSF1, TNF ligand superfamily member 1) a member of the TNF ligand superfamily and a potent lymphoid factor, is mainly produced by activated T and B lymphocytes and leukocytes. TNF-beta is also produced by fibroblasts, astrocytes, myeloma cells, endothelial cells and epithelial cells. TNF-beta mediates inflammatory and immune responses, has cytotoxic activity on tumor cells and other target cells and shares many of the same bioactivities as TNF alpha, including regulation of cell proliferation, differentiation and apoptosis. TNF-beta is not species-specific and human TNF-beta is active on murine cells with a slightly reduced specific activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol LTA