Genetic Strategies: Knockout Validated: Kif2a Antibody [NB500-180] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Kif2a knockout (KO) HEK293T cell line. PVDF membrane was ...read more
Western Blot: Kif2a Antibody [NB500-180] - Analysis of Kif2a in Hela, 3T3 and PC12 lysate.
Immunocytochemistry/ Immunofluorescence: Kif2a Antibody [NB500-180] - Kif2a antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Simple Western: Kif2a Antibody [NB500-180] - Lane view shows a specific band for Kif2a in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
This Kif2a antibody is useful for Western blot, Immunoprecipitation, and Immunocytochemistry/Immunofluorescence. A band at approx. 110 kDa can be detected by Western blot.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
110 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NB500-180 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Unpurified
Alternate Names for Kif2a Antibody
HK2
KIF2kinesin-2
kinesin heavy chain member 2
kinesin heavy chain member 2A
Kinesin, heavy chain, 2
Kinesin-2
kinesin-like protein KIF2A
KNS2
Background
Kif2a (Kinesin-like protein KIF2A or Kinesin-2) belongs to Kinesin-13 family that includes Kif2a, Kif2b and Kif2c members which are classified as microtubule depolymerases with potential of depolymerizing microtubules at their ends. Among these proteins, Kif2a specifically localizes to centrosomes during mitotic cell division and is essential to normal congress of chromosomes at the metaphase plate, normal spindle dynamics/turnover and for the formation of bipolar mitotic spindles. Kif2a gets localized to the spindle microtubules and spindle poles from prophase to metaphase. Kif2a-PSRC1 interactions leads to its recruitment to mitotic spindles, PLK1 kiase activity is required for Kif2a targeting to spindle microtubules and spindle poles. siRNA knockdown of Kif2a in human cancer cells or Kif2a blocking with antibody addition to Xenopus eggs leads to monopolar spindles formation and cell cycle block suggesting that Kif2a is essential for bipolar spindle assembly and normal progression of mitosis. Kif2a has been shown to be essential for proper neuronal outgrowth and its depletion caused neuronal migratory delay by changing microtubule dynamics around the cell body suggesting that Kif2a is implicated in non-mitotic cells also. Moreover, Kif2a has been established as a plus end-directed microtubule-dependent motor essential for normal brain development and that it regulates microtubule dynamics during axonal growth.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Kif2a Antibody (NB500-180). (Showing 1 - 2 of 2 FAQs).
Can you provide the immunogen sequence for the Kif2a Antibody Cat. No. NB500-180? If considered proprietary, can you provide the % homology with drosophila?
The exact immunogen sequence of NB500-180 is proprietary, but it falls within amino acids 1-120 of human Kif2A (Accession #: O00139). Based on an alignment of the immunogen with the Drosophila melanogaster sequence, I am getting a homology of about 25%.
We receive an enquiry about the following antibody: Anti-Kif2a antibody (NB 500-180). The customer would like to know the immunogen of the antibody. If this information is not available, I would appreciate if you could at least give the amino acids range at the N-ter recognized by the antibody.
Unfortunately many of our immunogen sequences are proprietary and we are unable to disclose the exact amino acid range. However, we can confirm you that the immunogen which was used to generate our Kif2a antibody with catalogue number NB500-180 is derived from a region within the first 150 amino acids of the protein.
Kif2a and MT-Destabilization during Mitosis Kif2a belongs to the Kinesin-13 microtubule depolymerase family that includes members Kif2a, Kif2b, and Kif2c. These proteins are capable of depolymerizing microtubules (MTs) at their ends. During mitotic cell division, Kif2a specifically localizes to... Read full blog post.
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![Knockout Validated: Kif2a Antibody [NB500-180] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Kif2a knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:10000 of Rabbit Anti-Human Kif2a Polyclonal Antibody (Catalog # NB500-180) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Kif2a at approximately 100 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Kif2a-Antibody-Knockout-Validated-NB500-180-img0007.jpg "Knockout Validated: Kif2a Antibody [NB500-180] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Kif2a knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:10000 of Rabbit Anti-Human Kif2a Polyclonal Antibody (Catalog # NB500-180) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Kif2a at approximately 100 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![Western Blot: Kif2a Antibody [NB500-180] - Analysis of Kif2a in Hela, 3T3 and PC12 lysate.](http://images.novusbio.com/fullsize/Kif2a-Antibody-Western-Blot-NB500-180-img0003.jpg "Western Blot: Kif2a Antibody [NB500-180] - Analysis of Kif2a in Hela, 3T3 and PC12 lysate.")
![Immunocytochemistry/Immunofluorescence: Kif2a Antibody [NB500-180] - Kif2a antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Kif2a-Antibody-Immunocytochemistry-Immunofluorescence-NB500-180-img0005.jpg "Immunocytochemistry/Immunofluorescence: Kif2a Antibody [NB500-180] - Kif2a antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![Simple Western: Kif2a Antibody [NB500-180] - Lane view shows a specific band for Kif2a in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Kif2a-Antibody-Simple-Western-NB500-180-img0006.jpg "Simple Western: Kif2a Antibody [NB500-180] - Lane view shows a specific band for Kif2a in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")