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Goat anti-Chicken IgM Fc Secondary Antibody [HRP]

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Product Details

Summary
Reactivity ChSpecies Glossary
Applications WB, DB, ELISA, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
LYOPH

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Goat anti-Chicken IgM Fc Secondary Antibody [HRP] Summary

Description
The lyophilized conjugate is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Immunogen
Purified IgM isolated from chicken serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in chicken serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Clonality
Polyclonal
Host
Goat
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • Dot Blot
  • ELISA
  • Immunocytochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Reactivity Notes

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this immunoconjugate has not been tested in detail.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.2)
Preservative
No Preservative
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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