![Western Blot: IgG2b Antibody (RM108) [Biotin] [NBP2-62009B] - Western blot of nonreduced(-) and reduced(+) mouse IgG2b (20 ng/lane), using 0.2 ug/mL of NBP2-62009B. This antibody reacts to nonreduced IgG2b (~150 kDa) much stronger than the reduced gamma 2b form (~50 kDa).](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2b-Secondary-Antibody-RM108-Biotin-Western-Blot-NBP2-62009B-img0002.jpg "Western Blot: IgG2b Antibody (RM108) [Biotin] [NBP2-62009B] - Western blot of nonreduced(-) and reduced(+) mouse IgG2b (20 ng/lane), using 0.2 ug/mL of NBP2-62009B. This antibody reacts to nonreduced IgG2b (~150 kDa) much stronger than the reduced gamma 2b form (~50 kDa).")
| Reactivity | MuSpecies Glossary |
| Applications | WB, ELISA |
| Clone | RM108 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Biotin |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Mouse IgG2b |
| Specificity | This antibody reacts to the Fc region of mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This primary antibody can also be used as a secondary antibody. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 50% Glycerol, 1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![ELISA: IgG2b Antibody (RM108) [Biotin] [NBP2-62009B] - ELISA of mouse immunoglobulins shows NBP2-62009B reacts to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with different immunoglobulins. NBP2-62009B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2b-Secondary-Antibody-RM108-Biotin-ELISA-NBP2-62009B-img0003.jpg "ELISA: IgG2b Antibody (RM108) [Biotin] [NBP2-62009B] - ELISA of mouse immunoglobulins shows NBP2-62009B reacts to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with different immunoglobulins. NBP2-62009B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.")
![ELISA: IgG2b Antibody (RM108) [Biotin] [NBP2-62009B] - A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of NBP2-62009B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2b-Secondary-Antibody-RM108-Biotin-ELISA-NBP2-62009B-img0005.jpg "ELISA: IgG2b Antibody (RM108) [Biotin] [NBP2-62009B] - A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of NBP2-62009B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.")
