Reactivity | ChSpecies Glossary |
Applications | WB, ELISA, IHC |
Clonality | Polyclonal |
Host | Goat |
Conjugate | HRP |
Format | Pre-adsorbed |
Concentration | LYOPH |
Description | Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Goat Serum, Chicken IgG and Chicken Serum |
Immunogen | Goat anti-Chicken IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) was produced by repeated immunization with Chicken IgG whole molecule in goat. |
Specificity | No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins. |
Isotype | IgG |
Clonality | Polyclonal |
Host | Goat |
Purity | Multi-step |
Dilutions |
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Application Notes | This product has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly). |
Readout System |
Storage | Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative | 0.01% Gentamicin Sulfate |
Concentration | LYOPH |
Purity | Multi-step |
Reconstitution Instructions | Reconstitute with 1.0 ml deionized water (or equivalent). |
Research Areas for IgG (H+L) Antibody (NBP1-73733)Find related products by research area.
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