![Western Blot: Rabbit anti-Chicken IgG Fc Secondary Antibody [Alkaline Phosphatase] [NBP1-72633] - Lane 1: Chicken IgG whole molecule. Lane 2: Chicken IgG F(c) Lane 3: Chicken IgG Fab Fragment. Lane 4: Chicken IgM Whole Molecule. Lane 5: Chicken Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Chicken IgG F(c) (RABBIT) Antibody 1:2,000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgG and Serum, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit-anti-Chicken-IgG-Fc-Secondary-Antibody-[Alkaline-Phosphatase]-Western-Blot-NBP1-72633-img0004.jpg "Western Blot: Rabbit anti-Chicken IgG Fc Secondary Antibody [Alkaline Phosphatase] [NBP1-72633] - Lane 1: Chicken IgG whole molecule. Lane 2: Chicken IgG F(c) Lane 3: Chicken IgG Fab Fragment. Lane 4: Chicken IgM Whole Molecule. Lane 5: Chicken Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Chicken IgG F(c) (RABBIT) Antibody 1:2,000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgG and Serum, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.")
| Reactivity | ChSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Alkaline Phosphatase |
| Description | Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Chicken IgG, Chicken IgG F(c) and Chicken Serum |
| Immunogen | Chicken IgG F(c) fragment |
| Specificity | No reaction was observed against Chicken IgG F(ab). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Multi-step |
| Dilutions |
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| Application Notes | This product has been tested by ELISA. This product has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:9,000 of the reconstitution concentration is suggested for this product. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.1% Sodium Azide |
| Purity | Multi-step |
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