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Donkey IgG F(ab')2 Isotype Control [Biotin]

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Product Details

Summary
Reactivity DoSpecies Glossary
Applications WB, Flow, IHC
Host
Donkey
Conjugate
Biotin
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Donkey IgG F(ab')2 Isotype Control [Biotin] Summary

Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey IgG, anti-Donkey IgG F(ab')2 and anti-Donkey Serum
Specificity
No reaction was observed against anti-Donkey IgG F(c) or anti-Pepsin.
Isotype
IgG
Host
Donkey
Purity
Multi-step

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Western Blot
Application Notes
This product reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Multi-step
Reconstitution Instructions
Reconstitute with 1.0 ml deionized water (or equivalent).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Video Protocols

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