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Donkey anti-Goat IgG Secondary Antibody [NL557]

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Detection of human EGFR by incubating cells with 5 μg/mL of Goat anti-Human EGFR polyclonal antibody (AF231) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Goat IgG ...read more

Product Details

Summary
Reactivity GtSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Donkey
Conjugate
NL557

Donkey anti-Goat IgG Secondary Antibody [NL557] Summary

Additional Information
NorthernLights 557 Fluorochrome-labeled Antibody, X Absorbed
Immunogen
Goat IgG
Specificity
Goat IgG (H+L)
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Immunocytochemistry
Publications
Read Publications using
NL001 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Goat IgG Secondary Antibody [NL557]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (NL001)(36)

We have publications tested in 3 confirmed species: Human, Mouse, Rats.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Secondary Antibody.


Filter By Application
Flow Cytometry
(5)
ICC
(16)
IHC
(4)
IHC-Fr
(2)
IHC-P
(3)
Secondary Antibody
(1)
All Applications
Filter By Species
Human
(20)
Mouse
(7)
Rats
(1)
All Species
Showing Publications 1 - 10 of 36. Show All 36 Publications.
Publications using NL001 Applications Species
Lindsey E. Padgett, Paola M. Marcovecchio, Claire E. Olingy, Daniel J. Araujo, Kathleen Steel, Huy Q. Dinh et al. Nonclassical monocytes potentiate anti-tumoral CD8+ T cell responses in the lungs Frontiers in Immunology 2023-06-22 [PMID: 37426658]
L Li, X Si, J Yang, M Lei, H Liu, J Ruan, H Fu, W Li, H Yang, X Lei, H Sang Generation of a human iPSC line (CIBi014-A) from a patient with Parkinson&#039;s disease carrying a novel heterozygotic PARK8 (LRRK2) mutation Stem Cell Research, 2022-12-09;66(0):102995. 2022-12-09 [PMID: 36528012] (ICC, Human) ICC Human
H Lin, SQ Ye, ZH Xu, JS Penaloza, M Aljuhani, T Vetter, MT Zhao, KL McBride Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD) Stem Cell Research, 2022-10-31;65(0):102958. 2022-10-31 [PMID: 36343514] (ICC, Human) ICC Human
J Yang, L Jin, HS Kim, F Tian, Z Yi, K Bedi, M Ljungman, M Pasca di M, H Crawford, J Shi KDM6A Loss Recruits Tumor-Associated Neutrophils and Promotes Neutrophil Extracellular Trap Formation in Pancreatic Cancer Cancer Research, 2022-11-15;0(0):OF1-OF14. 2022-11-15 [PMID: 36306422]
Z Jiang, XJ Gu, WM Su, QQ Duan, JY Lin, B Cao, HF Shang, YP Chen Production of a human iPSC line from an early-onset Parkinson&#039;s disease patient with a novel CHCHD2 gene truncated mutation Stem Cell Research, 2022-08-04;64(0):102881. 2022-08-04 [PMID: 35944313] (Flow Cytometry, ICC, Human) Flow Cytometry, ICC Human
Y Sun, J Fu, J Yang, J Zhao, J Rong Generation of a RRAGA knockout human iPSC line GIBHi002-A-5 using CRISPR/Cas9 technology Oncogene, 2022-07-11;63(0):102859. 2022-07-11 [PMID: 35870248] (Flow Cytometry, ICC, Human) Flow Cytometry, ICC Human
Y Shi, S Wu, B Yang, B Yu, Y Yan, J Yang, M Chu, X Hu Generation of a human iPSC line CIBi011-A from amniocytes of a healthy fetus Stem Cell Research, 2022-05-04;62(0):102801. 2022-05-04 [PMID: 35609349] (ICC, Human) ICC Human
X Hu, S Xiong, X Zhou, L Sun Generation of a human induced pluripotent stem cell line FMUPDCi001-A from a patient with mental retardation, autosomal recessive 36 (MRT36) carrying the variants c.219dupA and c.587C�&gt;�T in ADAT3 Stem Cell Research, 2022-04-04;61(0):102777. 2022-04-04 [PMID: 35405382] (ICC, Human) ICC Human
DA Clark, A Reihani, JL Arredondo, K Ask, WG Foster CD200S-positive granulated lymphoid cells in endometrium appear to be CD56-positive uterine NK cells Journal of reproductive immunology, 2022-01-14;150(0):103477. 2022-01-14 [PMID: 35051828] (IHC, Human) IHC Human
J Zhao, C Huangfu, Z Chang, W Zhou, AT Grainger, Z Liu, W Shi Inflammation and enhanced atherogenesis in the carotid artery with altered blood flow in an atherosclerosis-resistant mouse strain Physiological Reports, 2021-06-01;9(11):e14829. 2021-06-01 [PMID: 34110700] (IHC, Mouse) IHC Mouse
Show All 36 Publications.

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CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Bioinformatics

Gene Symbol IGHG1