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HspBP1 Antibody (OTI1D5)

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Western Blot: HspBP1 Antibody (OTI1D5) [NBP2-01168] - Analysis of extracts (35ug) from 9 different cell lines by using anti-HspBP1 monoclonal antibody.
Immunohistochemistry-Paraffin: HspBP1 Antibody (OTI1D5) [NBP2-01168] - Staining of paraffin-embedded Human pancreas tissue using anti-HspBP1 mouse monoclonal antibody.
Flow Cytometry: HspBP1 Antibody (OTI1D5) [NBP2-01168] - HEK293T cells transfected with either HspBP1 Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ...read more
Western Blot: HspBP1 Antibody (OTI1D5) [NBP2-01168] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HspBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunohistochemistry-Paraffin: HspBP1 Antibody (OTI1D5) [NBP2-01168] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HspBP1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HspBP1 Antibody (OTI1D5) [NBP2-01168] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HspBP1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HspBP1 Antibody (OTI1D5) [NBP2-01168] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HspBP1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HspBP1 Antibody (OTI1D5) [NBP2-01168] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HspBP1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HspBP1 Antibody (OTI1D5) [NBP2-01168] - Staining of paraffin-embedded Human endometrium tissue using anti-HspBP1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HspBP1 Antibody (OTI1D5) [NBP2-01168] - Staining of paraffin-embedded Human lymphoma tissue using anti-HspBP1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Flow, IHC
Clone
OTI1D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.43 mg/ml

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HspBP1 Antibody (OTI1D5) Summary

Immunogen
Full length human recombinant protein of human HSPBP1(NP_036399) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSPBP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
39.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-01168.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.43 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HspBP1 Antibody (OTI1D5)

  • FES1
  • Heat shock protein-binding protein 1
  • Hsp70 binding protein 1
  • hsp70 interacting protein
  • hsp70-binding protein 1
  • Hsp70-binding protein 2
  • Hsp70-interacting protein 1
  • Hsp70-interacting protein 2
  • HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1
  • HSPBP
  • HspBP1
  • hspBP2

Background

Heat shock protein binding protein is an Hsp70-interacting protein that was first isolated from a human heart cDNA library using the yeast two-hybrid system. HspBP1 binds the ATPase domain of Hsp70, and inhibits Hsp70 chaperone activity. More recently HspBP1 was found to promote nucleotide dissociation from Hsc70 and is capable of altering the confirmation of the Hsp70 ATPase domain. HspBP1 is capable of regulating any Hsp70 activity that requires ATPase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HspBP1 Antibody (NBP2-01168)(1)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HSPBP1
Entrez