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HLA ABC Antibody (W6/32) [Janelia Fluor® 525]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready, IHC-P (-)
Clone
W6/32
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 525

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-64775AF350
Alexa Fluor 405
NB100-64775AF405
Alexa Fluor 488
NB100-64775AF488
Alexa Fluor 532
NB100-64775AF532
Alexa Fluor 594
NB100-64775AF594
Alexa Fluor 647
NB100-64775AF647
Alexa Fluor 700
NB100-64775AF700
Alexa Fluor 750
NB100-64775AF750
Allophycocyanin
NB100-64775APC
Biotin
NB100-64775B
DyLight 350
NB100-64775UV
DyLight 405
NB100-64775V
DyLight 488
NB100-64775G
DyLight 550
NB100-64775R
DyLight 594
NB100-64775DL594
DyLight 650
NB100-64775C
DyLight 680
NB100-64775FR
DyLight 755
NB100-64775IR
FITC
NB100-64775F
HRP
NB100-64775H
Janelia Fluor 549
NB100-64775JF549
Janelia Fluor 585
NB100-64775JF585
Janelia Fluor 635
NB100-64775JF635
Janelia Fluor 646
NB100-64775JF646
Janelia Fluor 669
NB100-64775JF669
PE
NB100-64775PE
PerCP
NB100-64775PCP
Unconjugated
NB100-64775
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HLA ABC Antibody (W6/32) [Janelia Fluor® 525] Summary

Immunogen
Purified human tonsil lymphocyte membranes
Specificity
NB100-64775 recognizes an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains (6). MHC class I glycoproteins are found on the surface of almost all nucleated somatic cells. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HLA-A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Mouse reactivity reported in scientific literature (Parasar P et al).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for HLA ABC Antibody (W6/32) [Janelia Fluor® 525]

  • A-10 alpha chain
  • FLJ26655
  • HLA class I histocompatibility antigen, A-1 alpha chain
  • HLA class I histocompatibility antigen, A-28 alpha chain
  • HLA class I histocompatibility antigen, A-9 alpha chain
  • HLAA
  • major histocompatibility complex, class I, A
  • MHC class I antigen A*1
  • MHC class I antigen A*11
  • MHC class I antigen A*80

Background

HLA CLass I is involved in the presentation of foreign antigens to the immune system. HLA-class I antigens are widely distributed on human nucleated cells. The intensity of HLA-ABC maybe altered in pathological states e.g. malignant cells may loose HLA-ABC, whereas hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may show enhanced expression of HLA-ABC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HLA-A