![Immunohistochemistry: Histone H3.1 Antibody (27F2) [NBP3-26228] - Image of Histone H3.1 Antibody (27F2) diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26228_rabbit-histone-h3-1-mab-27f2-2722024857331.jpg "Immunohistochemistry: Histone H3.1 Antibody (27F2) [NBP3-26228] - Image of Histone H3.1 Antibody (27F2) diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clone | 27F2 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant monoclonal antibody expressed in HEK293F cells |
| Immunogen | Synthesized peptide [UniProt P68431] |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | HIST1H3E |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20 to -70C. Avoid freeze-thaw cycles. |
| Buffer | PBS, pH 7.4, 150mM NaCl and 50% glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7)](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7)")
Secondary Antibodies |
Isotype Controls |
Research Areas for Histone H3.1 Antibody (NBP3-26228)Find related products by research area.
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H3.1 - A core histone essential for genome storage and organization Histones are the main protein component of chromatin and are essential for the storage and compaction of the genome. DNA wraps around histone oligomers to make up nucleosomes, the individual subunits of chromatin. By altering the accessibility of t... Read full blog post. |
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| Gene Symbol | HIST1H3E |
![Immunohistochemistry: Histone H3.1 Antibody (27F2) [NBP3-26228] - Image of Histone H3.1 Antibody (27F2) diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26228_rabbit-histone-h3-1-mab-27f2-27220248471915.jpg "Immunohistochemistry: Histone H3.1 Antibody (27F2) [NBP3-26228] - Image of Histone H3.1 Antibody (27F2) diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: Histone H3.1 Antibody (27F2) [NBP3-26228] - Positive Western Blot detected in Jurkat whole cell lysate. All lanes: Histone H3.1 Antibody at 1.5ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 KDa Observed band size: 15 KDa](http://images.novusbio.com/fullsize/nbp3-26228_rabbit-histone-h3-1-mab-27f2-282202411485848.jpg "Western Blot: Histone H3.1 Antibody (27F2) [NBP3-26228] - Positive Western Blot detected in Jurkat whole cell lysate. All lanes: Histone H3.1 Antibody at 1.5ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 KDa Observed band size: 15 KDa")
![Immunocytochemistry/Immunofluorescence: Histone H3.1 Antibody (27F2) [NBP3-26228] - Staining of Hela cells with Histone H3.1 Antibody (27F2) at 1:93, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26228_rabbit-histone-h3-1-mab-27f2-2822024132362.jpg "Immunocytochemistry/Immunofluorescence: Histone H3.1 Antibody (27F2) [NBP3-26228] - Staining of Hela cells with Histone H3.1 Antibody (27F2) at 1:93, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).")
![Flow Cytometry: Histone H3.1 Antibody (27F2) [NBP3-26228] - Overlay histogram showing Hela cells stained with Histone H3.1 Antibody (27F2) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26228_rabbit-histone-h3-1-mab-27f2-282202414183685.jpg "Flow Cytometry: Histone H3.1 Antibody (27F2) [NBP3-26228] - Overlay histogram showing Hela cells stained with Histone H3.1 Antibody (27F2) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")