His Tag Antibody (AD1.1.10) - Non-Recombinant Monoclonal

A his-tag (also known as histidine tag or polyhistidine tag) is a common epitope tag that typically consists of at least 6 histidine residues fused to either the carboxyl (C-) or amino (N-) terminus of a targeted recombinant protein to facilitate its purification and detection (1). The most common his-tag is the hexahistidine (His6/6-His) tag which has a theoretical molecular weight of 0.8kda (1). The histidine residues readily interact with transition metal ions such as Co2+, Ni2+, Cu2+, and Zn2+, making immobilized metal-affinity chromatography (IMAC) the preferred technique for his-tag purification (1, 2). Metal ions are immobilized and bound to by His-tags in the IMAC column via the histidine imidazole ring. The tagged protein can be eluted off the column by washing with buffers containing a low concentration of imidazole (1, 2). Due to its relatively small size, low immunogenicity, versatility under denaturing conditions, and minimal interference with the structure and function of proteins, the his-tag is one of the most widely used tags for protein purification (1-3).

References

1. Malhotra, A. (2009). Tagging for protein expression. Methods in Enzymology, Guide to Protein Purification, 2nd Edition, 463, 239-258. https://doi.org/10.1016/s0076-6879(09)63016-0

2. Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60(5), 523-533. https://doi.org/10.1007/s00253-002-1158-6

3. Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V., ... Chruszcz, M. (2018). Impact of an N-terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768. https://doi.org/10.1021/acsomega.7b01598

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ELISA, ICC/IF, IP, WB.

Showing Publications 1 - 5 of 5.

Publications using NB100-64768	Applications	Species
Jang S, Song J, Kim N et al. Development of an antibody-like T-cell engager based on VH-VL heterodimer formation and its application in cancer therapy Biomaterials 2021-03-28 [PMID: 33774526] Details: Citation using the Non-Recombinant Monoclonal format of this antibody.
Davids BJ, Liu CM, Hanson EM et al. Identification of Conserved Candidate Vaccine Antigens in the Surface Proteome of Giardia lamblia Infect Immun 2019-04-10 [PMID: 30962402] (WB, Human) Details: Citation using the Non-Recombinant Monoclonal format of this antibody.	WB	Human
Puerta-Guardo H, Glasner DR, Espinosa DA et al. Flavivirus NS1 Triggers Tissue-Specific Vascular Endothelial Dysfunction Reflecting Disease Tropism Cell Rep 2019-02-07 [PMID: 30726741] (ICC/IF) Details: Citation using the Alexa Fluor 647 format of this antibody.	ICC/IF
Kim MJ, Vargas MR, Harlan BA et al. Nitration and Glycation Turn Mature NGF into a Toxic Factor for Motor Neurons: A Role for p75(NTR) and RAGE Signaling in ALS Antioxid Redox Signal 2017-05-25 [PMID: 28537420] (IP, WB, Mouse) Details: Citation using the Non-Recombinant Monoclonal format of this antibody.	IP, WB	Mouse
Tseng FJ, Liu YK, Chung YS et al. A fusion protein composed of receptor binding domain of vascular endothelial growth factor-A and constant region fragment of antibody: angiogenesis antagonistic activity Cytotechnology 2011-04-05 [PMID: 21461946] (ELISA) Details: Citation using the Non-Recombinant Monoclonal format of this antibody.	ELISA

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	WB	Drosophila	07/10/2017	View Summary Application Western Blot Sample Tested S2 lysate (with overexpressed recombinant protein) Species Drosophila Lot D-1
Enlarge	5 reviewed by: Eric Wang	WB	Drosophila	04/13/2017	View Summary Application Western Blot Sample Tested 20 ug whole cell lysate Species Drosophila Lot c-4

Array NB100-64768

• His Tag Antibodies