Novus Biologicals products are now on bio-techne.com

HIPPI Recombinant Protein Antigen

Images

 
There are currently no images for HIPPI Protein (NBP1-84788PEP).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications AC

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

HIPPI Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human IFT57.

Source: E. coli

Amino Acid Sequence: EGTGEVVLERGPGAAYHMFVVMEDLVEKLKLLRYEEEFLRKSNLKAPSRHYFALPTNPGEQFYMFCTLAAWLINKAGRPFEQPQEYDDPN

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
IFT57
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10 - 100 molar excess
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-84788.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

For further blocking peptide related information and a protocol, click here.

Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Alternate Names for HIPPI Recombinant Protein Antigen

  • Dermal papilla-derived protein 8
  • DERP8
  • ESRRBL1huntingtin interacting protein-1 interacting protein
  • estrogen-related receptor beta like 1
  • Estrogen-related receptor beta-like protein 1
  • FLJ10147
  • HIPPIHIP1-interacting protein
  • intraflagellar transport 57 homolog (Chlamydomonas)
  • intraflagellar transport protein 57 homolog
  • MHS4R2HIP1 protein interactor

Background

HIPPI is required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway . Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and tri

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-204
Species: Ba, Hu, Mu, Rt(-)
Applications: ICC/IF, IP, WB
NBP3-13106
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP3-03807
Species: Hu, Mu, Rt
Applications: ICC/IF, IP, WB
NB100-56116
Species: Ma, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
NB100-56094
Species: Hu
Applications: IHC, IHC-P, IP, WB
H00006532-D01P
Species: Hu, Mu
Applications: WB
NB100-2475
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-Fr, PEP-ELISA, WB
NB100-56565
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
NBP2-02009
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-757
Species: Hu
Applications: ChIP, IHC, IHC-P, WB
DBD00
Species: Hu
Applications: ELISA
NBP2-55756
Species: Hu
Applications: IHC, IHC-P
NBP1-90944
Species: Hu
Applications: IHC, IHC-P, WB
375-TL
Species: Hu
Applications: BA
NB100-56118
Species: Ca, Ma, Hu, Mu, Rt
Applications: Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-98642
Species: Hu
Applications: IHC, IHC-P, IP, WB
H00057728-B01P
Species: Hu, Mu
Applications: ICC/IF, WB
NBP2-67187
Species: Hu, Mu, Rt, Ze
Applications: ICC/IF, IHC, IHC-P, IP, WB

Publications for HIPPI Protein (NBP1-84788PEP) (0)

There are no publications for HIPPI Protein (NBP1-84788PEP).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HIPPI Protein (NBP1-84788PEP) (0)

There are no reviews for HIPPI Protein (NBP1-84788PEP). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for HIPPI Protein (NBP1-84788PEP) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional HIPPI Products

Blogs on HIPPI

There are no specific blogs for HIPPI, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Caspase-8 Antibody
NB100-56116

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

The molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) x Volume (L) x Molecular Weight (g/mol)

As an example, if the molecular weight of a compound is 197.13 g/mol and the desired concentration is 10 mM for 10 ml of water based stock solution, the required mass would be = 19.713 (value determined by this calculator).

=
x
x
g/mol

Review this Product

Be the first to review our HIPPI Recombinant Protein Antigen and receive a gift card or discount.

Bioinformatics

Gene Symbol IFT57