Human HBsAg ELISA Kit (Colorimetric) Summary
| Standard Curve Range |
0.31 - 20 ng/mL |
| Sensitivity |
0.19 ng/mL |
| Assay Type |
Sandwich-ELISA |
| Inter-Assay |
%CV < 5.81 |
| Intra-Assay |
%CV < 6.02 |
| Spike Recovery |
86 - 98% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
S |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Biotinylated Detection Antibody Diluent
- Concentrated Biotinylated Detection Antibody
- Concentrated HRP Conjugate
- Concentrated Wash Buffer
- HRP Conjugate Diluent
- Micro ELISA Plate
- Plate Sealer
- Reference Standard & Sample Diluent
- Reference Standard
- Stop Solution
- Substrate Reagent
|
Alternate Names for Human HBsAg ELISA Kit (Colorimetric)
Background
Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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Blogs on HBsAg
![ELISA: Human HBsAg ELISA Kit (Colorimetric) [NBP3-11804] - Samples were spiked with high concentrations of Human HBsAg and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-11804_human-hbsag-elisa-kit-colorimetric-132202416175645.jpg "ELISA: Human HBsAg ELISA Kit (Colorimetric) [NBP3-11804] - Samples were spiked with high concentrations of Human HBsAg and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human HBsAg ELISA Kit (Colorimetric) [NBP3-11804] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-11804_human-hbsag-elisa-kit-colorimetric-4320249133282.jpg "ELISA: Human HBsAg ELISA Kit (Colorimetric) [NBP3-11804] - Standard Curve Reference")