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HA Tag Antibody (912426) [Unconjugated]

Images

 
Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal HA-tagged LGI-2, Internal Sequence HA-tagged CIGALT, and C-terminal HA-tagged FBLN. PVDF membrane was probed with 0.5 ...read more
HA Tag was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-HA Tag Monoclonal Antibody (Catalog # MAB6875) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more
HEK293 human embryonic kidney cell line transfected with HA-tagged proteins was stained with Mouse Anti-HA Monoclonal Antibody (Catalog # MAB6875, filled histogram) or isotype control antibody (Catalog # MAB002, open ...read more

Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications WB, CyTOF-ready, Flow, ICC/IF
Clone
912426
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HA Tag Antibody (912426) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
HA Tag peptide
Accession # ABB51961
Specificity
Detects N-terminal,C-terminal, and Internal Sequence HA-tagged proteins in Western blot.
Details of Functionality
None<br /><br />None<br /><br />None<br /><br />
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.5 ug/mL
Reviewed Applications
Read 1 Review rated 3
using
MAB6875 in the following applications:

Publications
Read Publication using MAB6875.

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HA Tag Antibody (912426) [Unconjugated]

  • HA Epitope Tag
  • HA Tag
  • HA
  • Hemagglutinin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HA Tag Antibody (MAB6875)(1)

Review for HA Tag Antibody (MAB6875) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB6875:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot HA Tag MAB6875
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3
reviewed by:
Verified Customer
WB Human 10/19/2017
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Summary

ApplicationWestern Blot
Sample Tested293T lysate
SpeciesHuman
LotCJDS021710A

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HA Tag Antibody (MAB6875). (Showing 1 - 1 of 1 FAQ).

  1. We are looking for a NON-RABBIT polyclonal anti-HA (hemagglutinin - YPYDVPDYA ) antibody for immunohistochemistry that works in 4% paraformaldehyde-fixed tissue. We have a transgenic mouse expressing an HA-tagged protein and want to perform double-labeling with another antibody made in rabbit. We have had no luck using mouse or rat monoclonal anti-HA antibodies. The non-rabbit polyclonal antibody would preferably be made in goat, although if you have other species let us know. It could be unconjugated or conjugated (eg fluorochrome or HRP). However, we are only interested in a product with demonstrated effectiveness for IHC in a published paper. Do you have any such products?
    • We do have other host species for this target and some that have been mentioned in publications. Unfortunately for our goat antibodies against this tag none of them have been validated in IHC-P, and for two we have shown they do not work. Please see this link to our goat anti-HA Epitope Tag antibodies. Since they work in ICC/IF I would assume as long as the tag is expressed in your tissues that you should still pick it up. In this case we have an Innovators Reward Program you can take advantage of for testing a new application to save some money. Here are the chicken anti-HA Epitope Tag antibodies. None of them have been tested in IHC-P as before, or that would be indicated with a positive result and the application on the datasheet or a negative results with the (-) beside the application. Another option for you as well would be going with a Rabbit polyclonal that we have available that has been well publicized and reviewed by customers (cat# NB600-363). I know you plan on double staining, but one way around this would be to use your other antibody and incubate with the secondary for detection first. After doing that you could have this one directly conjugated to your preferred detection probe and then pick up both of their signals since you would already have bound secondary to your first rabbit primary, there would not be any non-specific binding.

Secondary Antibodies

 

Isotype Controls

Additional HA Tag Products

Array MAB6875

Blogs on HA Tag.

Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein
By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto...  Read full blog post.

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Contact Information

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Recent Reviews

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Verified Customer
10/19/2017
Application: WB
Species: Human

Bioinformatics

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