![Western Blot: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753] - Western Blot Analysis of human heart tissue lysate using H1F0 antibody (AE-4) - Azide and BSA Free](http://images.novusbio.com/fullsize/Histone-H1-Antibody-AE-4-Azide-and-BSA-Free-Western-Blot-NBP2-47753-img0007.jpg "Western Blot: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753] - Western Blot Analysis of human heart tissue lysate using H1F0 antibody (AE-4) - Azide and BSA Free")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF |
| Clone | AE-4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45182). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. |
| Immunogen | Nuclei of human leukemia biopsy cells |
| Localization | Nuclear |
| Marker | Pan Nuclear Marker |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | H1-0 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 30 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 1mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Diseases for H1F0 Antibody (NBP2-47753)Discover more about diseases related to H1F0 Antibody (NBP2-47753).
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| Gene Symbol | H1-0 |
![Immunocytochemistry/Immunofluorescence: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753] - Immunofluorescence staining of PFA-fixed HeLa cells using H1F0 antibody (AE-4) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (green). Membrane stained by Phalloidin](http://images.novusbio.com/fullsize/Histone-H1-Antibody-AE-4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47753-img0008.jpg "Immunocytochemistry/Immunofluorescence: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753] - Immunofluorescence staining of PFA-fixed HeLa cells using H1F0 antibody (AE-4) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (green). Membrane stained by Phalloidin")
![Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753] - Human Tonsil stained with H1F0 antibody (AE-4) - Azide and BSA Free](http://images.novusbio.com/fullsize/Histone-H1-Antibody-AE-4-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47753-img0006.jpg "Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753] - Human Tonsil stained with H1F0 antibody (AE-4) - Azide and BSA Free")
![Flow Cytometry: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753] - Flow Cytometric Analysis of HeLa cells. H1F0 antibody (AE-4) - Azide and BSA Free followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Histone-H1-Antibody-AE-4-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47753-img0001.jpg "Flow Cytometry: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753] - Flow Cytometric Analysis of HeLa cells. H1F0 antibody (AE-4) - Azide and BSA Free followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).")
![Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with H1F0 antibody (AE-4) - Azide and BSA Free](http://images.novusbio.com/fullsize/Histone-H1-Antibody-AE-4-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47753-img0003.jpg "Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with H1F0 antibody (AE-4) - Azide and BSA Free")
![Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753] - Formalin-fixed, paraffin-embedded human Pancreas stained with H1F0 antibody (AE-4) - Azide and BSA Free](http://images.novusbio.com/fullsize/Histone-H1-Antibody-AE-4-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47753-img0005.jpg "Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753] - Formalin-fixed, paraffin-embedded human Pancreas stained with H1F0 antibody (AE-4) - Azide and BSA Free")
