![Western Blot of GFP antibody [Alkaline Phosphatase]. Lane 1: GFP. Lane 2: none. Load: 50 ng per lane.Primary antibody: none.Secondary antibody: Alkaline Phosphatase GFP secondary antibody at 1:1000 for 60 min at RT.Block for 30 min RT.Predicted/Observed size: 28 kDa for GFP. Other band(s): none.](http://images.novusbio.com/fullsize/GFP-Antibody-[Alkaline-Phosphatase]-Western-Blot-NB600-1502-img0004.jpg "Western Blot of GFP antibody [Alkaline Phosphatase]. Lane 1: GFP. Lane 2: none. Load: 50 ng per lane.Primary antibody: none.Secondary antibody: Alkaline Phosphatase GFP secondary antibody at 1:1000 for 60 min at RT.Block for 30 min RT.Predicted/Observed size: 28 kDa for GFP. Other band(s): none.")
| Reactivity | All-NASpecies Glossary |
| Applications | WB, Simple Western, DB, ELISA, IHC, KD, KO |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Alkaline Phosphatase |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB100-1678 | |||
| DyLight 488 | NBP1-69969 | |||
| FITC | NB100-1771 | |||
| FITC | NB100-1771SS | |||
| HRP | NB600-313 | |||
| Rhodamine | NBP1-97327-500ug | |||
| Texas Red | NB120-6660 | |||
| Unconjugated | NB100-1770 | |||
| Description | Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum |
| Immunogen | The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria. (Uniprot: P42212) |
| Specificity | No reaction was observed against Human, Mouse and Rat Serum Proteins. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This product is designed to detect GFP and its variants. This product Alkaline Phosphatase conjugated antibody has been tested by ELISA, dot blot, and western blot. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.1% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Dot Blot of GFP Antibody Alkaline Phosphatase Conjugated. Antigen: GFP. Load: Lane 1 - 100ng Lane 2 - 33.33ng Lane 3 - 11.11ng Lane 4 - 3.70ng Lane 5 - 1.23ng. Primary antibody: n/a. Secondary antibody: GFP Antibody [Alkaline Phosphatase]at 1:1,000 for 60 min at RT. Block for 60 min at RT. Visualized using Alkaline Phosphatase Substrate.](http://images.novusbio.com/fullsize/nb600-1502_goat-polyclonal-gfp-antibody-alkaline-phosphatase-23520231750160.jpg "Dot Blot of GFP Antibody Alkaline Phosphatase Conjugated. Antigen: GFP. Load: Lane 1 - 100ng Lane 2 - 33.33ng Lane 3 - 11.11ng Lane 4 - 3.70ng Lane 5 - 1.23ng. Primary antibody: n/a. Secondary antibody: GFP Antibody [Alkaline Phosphatase]at 1:1,000 for 60 min at RT. Block for 60 min at RT. Visualized using Alkaline Phosphatase Substrate.")
