![Western Blot: GAT-1/SLC6A1 Antibody [NBP3-13166] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAT-1/SLC6A1 antibody (NBP3-13166) diluted at 1:500.](http://images.novusbio.com/fullsize/GAT-1-SLC6A1-Antibody-Western-Blot-NBP3-13166-img0001.jpg "Western Blot: GAT-1/SLC6A1 Antibody [NBP3-13166] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAT-1/SLC6A1 antibody (NBP3-13166) diluted at 1:500.")
| Reactivity | Mu, Rt, Hu, Bv, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of mouse GAT-1/SLC6A1. The exact sequence is proprietary. |
| Predicted Species | Human (98%), Bovine (94%), Rhesus Macaque (98%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SLC6A1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 67 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for GAT-1/SLC6A1 Antibody (NBP3-13166)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SLC6A1 |
![Immunocytochemistry/Immunofluorescence: GAT-1/SLC6A1 Antibody [NBP3-13166] - GAT-1/SLC6A1 antibody detects GAT-1/SLC6A1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAT-1/SLC6A1 stained by GAT-1/SLC6A1 antibody (NBP3-13166) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/GAT-1-SLC6A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13166-img0004.jpg "Immunocytochemistry/Immunofluorescence: GAT-1/SLC6A1 Antibody [NBP3-13166] - GAT-1/SLC6A1 antibody detects GAT-1/SLC6A1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAT-1/SLC6A1 stained by GAT-1/SLC6A1 antibody (NBP3-13166) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Frozen: GAT-1/SLC6A1 Antibody [NBP3-13166] - GAT-1/SLC6A1 antibody detects GAT-1/SLC6A1 protein by immunohistochemical analysis. Samples: Frozen Sectioned adult mouse hippocampus. Green: GAT-1/SLC6A1 protein stained by GAT-1/SLC6A1 antibody (NBP3-13166) diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min](http://images.novusbio.com/fullsize/GAT-1-SLC6A1-Antibody-Immunohistochemistry-Frozen-NBP3-13166-img0005.jpg "Immunohistochemistry-Frozen: GAT-1/SLC6A1 Antibody [NBP3-13166] - GAT-1/SLC6A1 antibody detects GAT-1/SLC6A1 protein by immunohistochemical analysis. Samples: Frozen Sectioned adult mouse hippocampus. Green: GAT-1/SLC6A1 protein stained by GAT-1/SLC6A1 antibody (NBP3-13166) diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min")
![Immunohistochemistry-Paraffin: GAT-1/SLC6A1 Antibody [NBP3-13166] - GAT-1/SLC6A1 antibody detects GAT-1/SLC6A1 protein at cell membrane and cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. GAT-1/SLC6A1 antibody (NBP3-13166) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GAT-1-SLC6A1-Antibody-Immunohistochemistry-Paraffin-NBP3-13166-img0002.jpg "Immunohistochemistry-Paraffin: GAT-1/SLC6A1 Antibody [NBP3-13166] - GAT-1/SLC6A1 antibody detects GAT-1/SLC6A1 protein at cell membrane and cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. GAT-1/SLC6A1 antibody (NBP3-13166) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: GAT-1/SLC6A1 Antibody [NBP3-13166] - GAT-1/SLC6A1 antibody detects GAT-1/SLC6A1 protein at cell membrane and cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GAT-1/SLC6A1 antibody (NBP3-13166) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GAT-1-SLC6A1-Antibody-Immunohistochemistry-Paraffin-NBP3-13166-img0003.jpg "Immunohistochemistry-Paraffin: GAT-1/SLC6A1 Antibody [NBP3-13166] - GAT-1/SLC6A1 antibody detects GAT-1/SLC6A1 protein at cell membrane and cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GAT-1/SLC6A1 antibody (NBP3-13166) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")