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FoxO1/FKHR Antibody

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Western Blot: FoxO1/FKHR Antibody [NB100-2312] - Whole cell lysate (50 ug) from Hep-G2, HeLa, HEK293T, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-FOXO1a antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: FoxO1/FKHR Antibody [NB100-2312] - Whole cell lysate (50 ug) from Hep-G2, HeLa, HEK293T, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-FOXO1a antibody used for WB ...read more
Independent Antibodies: Immunoprecipitation: FoxO1/FKHR Antibody [NB100-2312] - Detection of human FOXO1a by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

        

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-2312AF350
Alexa Fluor 405
NB100-2312AF405
Alexa Fluor 488
NB100-2312AF488
Alexa Fluor 532
NB100-2312AF532
Alexa Fluor 594
NB100-2312AF594
Alexa Fluor 647
NB100-2312AF647
Alexa Fluor 700
NB100-2312AF700
Alexa Fluor 750
NB100-2312AF750
Allophycocyanin
NB100-2312APC
Biotin
NB100-2312B
CoraFluor 1
NB100-2312CL1
DyLight 350
NB100-2312UV
DyLight 405
NB100-2312V
DyLight 488
NB100-2312G
DyLight 550
NB100-2312R
DyLight 594
NB100-2312DL594
DyLight 650
NB100-2312C
DyLight 680
NB100-2312FR
DyLight 755
NB100-2312IR
FITC
NB100-2312F
HRP
NB100-2312H
Janelia Fluor 525
NB100-2312JF525
Janelia Fluor 549
NB100-2312JF549
Janelia Fluor 646
NB100-2312JF646
Janelia Fluor 669
NB100-2312JF669
PE
NB100-2312PE
PerCP
NB100-2312PCP
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FoxO1/FKHR Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 600 and the C-terminus (residue 652) of human Forkhead Box O1a using the numbering given in Swiss-Prot entry Q12778 (GeneID 2308).
Predicted Species
Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FOXO1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000- 1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID 25344740)
Control
FoxO1/FKHR Overexpression Lysate
Publications
Read Publications using
NB100-2312 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28467929).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FoxO1/FKHR Antibody

  • FKH1
  • FKHR
  • FKHRforkhead box protein O1
  • forkhead box O1
  • Forkhead box protein O1A
  • forkhead homolog in rhabdomyosarcoma
  • Forkhead in rhabdomyosarcoma
  • FoxO1
  • FOXO1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma

Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FoxO1/FKHR Antibody (NB100-2312)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, WB, Western Blot.


Filter By Application
ICC/IF
(1)
WB
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(2)
Rat
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-2312 Applications Species
Sharma P, Bhandari C, Agnihotri N Dietary n-3 and n-6 polyunsaturated fatty acids differentially modulate the adiponectin and leptin mediated major signaling pathways in visceral and subcutaneous white adipose tissue in high fat diet induced obesity Nutrition Research 2022-12-01 [PMID: 36689814] (Western Blot, Rat) Western Blot Rat
Liu Z, Du J, Ren J et al. miR-183-96-182 clusters alleviated ox-LDL-induced vascular endothelial cell apoptosis in vitro by targeting FOXO1 RSC Adv 2022-05-13 [PMID: 35547044]
Ying, K;Feng, W;Ying, W;Li, X;Xing, D;Sun, Y;Chen, Y;Sanders, P; Dietary salt initiates redox signaling between endothelium and vascular smooth muscle through NADPH oxidase 4 Redox Biology [PMID: 35378363] (Mouse) Mouse
Calvier L, Chouvarine P, Legchenko E et al. PPARy Links BMP2 and TGFb1 Pathways in Vascular Smooth Muscle Cells, Regulating Cell Proliferation and Glucose Metabolism. Cell Metab. 2017-05-02 [PMID: 28467929] (Mouse) Mouse
Savai R, Al-Tamari Hm, Sedding D et al. Pro-proliferative and inflammatory signaling converge on FoxO1 transcription factor in pulmonary hypertension. Nat. Med. 2014-10-26 [PMID: 25344740] (ICC/IF, WB, Human) ICC/IF, WB Human
van der Horst PH, Wang Y, Vandenput I et al. Progesterone inhibits epithelial-to-mesenchymal transition in endometrial cancer. PLoS One 2012-01-01 [PMID: 22295114]
Yin X, Pavone ME, Lu Z et al. Increased activation of the PI3K/AKT pathway compromises decidualization of stromal cells from endometriosis. J Clin Endocrinol Metab 2012-01-01 [PMID: 22072736]
Khatri S, Yepiskoposyan H, Gallo CA et al. FOXO3a regulates glycolysis via transcriptional control of tumor suppressor TSC1. J Biol Chem 2010-05-01 [PMID: 20371605]
Amin AR, Thakur VS, Gupta K et al. Restoration of p53 functions protects cells from concanavalin A-induced apoptosis. Mol Cancer Ther 2010-02-01 [PMID: 20124456]
Hoekstra AV, Sefton EC, Berry E et al. Progestins activate the AKT pathway in leiomyoma cells and promote survival. J Clin Endocrinol Metab 2009-05-01 [PMID: 19240153]
Show All 11 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FoxO1/FKHR Antibody (NB100-2312). (Showing 1 - 1 of 1 FAQs).

  1. I've bought 2 antibodies from novus biologicals (NB100-614, NB100-2312) and now i am optimizing them for IP. Which beads did you use, protein a or protein g beads? in some cases only one kind of beads work, i have now tried protein a beads. If possible it would be also very helpful which protocol you used for the IP? (buffers, volumes, incubation times?)
    • Protein A beads were used for IP for these products. We don't have a specific protocol. We recommend for NB100-2312 a dilution of 1 to 10 mcg/mg lysate. For NB100-614 the dilution is 2 to 5 ug/mg lysate. Please see our general IP protocol.

Control Lysate(s)

FoxO1/FKHR Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

Additional FoxO1/FKHR Products

Research Areas for FoxO1/FKHR Antibody (NB100-2312)

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Blogs on FoxO1/FKHR.

Check out the latest blog posts on FoxO1/FKHR.
FOXO1/FKHR (fork head in Rhabdomyosarcoma)
FOXO1 belongs to the very large Forkhead family of transcription factors which contain a conserved distinct DNA-binding domain known as the Forkhead Box, or FOX. The Forkhead domain is a 100 amino acid long motif capable of binding and bending DNA,...  Read full blog post.
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Bioinformatics

Gene Symbol FOXO1
Entrez
Uniprot