I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?

Name: FGF basic/FGF2/bFGF Antibody [Biotin]
Brand: Novus Biologicals
SKU: BAF233
Price: 519 USD

Question

Accepted Answer

Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have several antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our  NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, IHC
Clonality	Polyclonal
Host	Goat
Conjugate	Biotin
Concentration	LYOPH

Catalog# & Conjugate	Size	Price
Unconjugated AF-233-NA

Catalog# & Formulation	Size	Price
Standard Formulation AF-233-NA

Carrier Free AF-233-NA

Carrier Free AF-233-SP

Conjugate	Catalog #	Availability	Size	Price
Unconjugated	AF-233-NA

FGF basic/FGF2/bFGF Antibody [Biotin]

Species Glossary

Images

Product Details

Order Details

FGF basic/FGF2/bFGF Antibody [Biotin] Summary

Applications/Dilutions

Packaging, Storage & Formulations

Notes

Alternate Names for FGF basic/FGF2/bFGF Antibody [Biotin]

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for FGF basic/FGF2/bFGF Antibody (BAF233) (0)

Reviews for FGF basic/FGF2/bFGF Antibody (BAF233) (0)

Product General Protocols

Video Protocols

FAQs for FGF basic/FGF2/bFGF Antibody (BAF233). (Showing 1 - 1 of 1 FAQ).

Secondary Antibodies

Isotype Controls

Additional FGF basic/FGF2/bFGF Products

Blogs on FGF basic/FGF2/bFGF

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics

Immunogen	E. coli-derived recombinant human FGF basic/FGF2/bFGF Pro143-Ser288 Accession # P09038
Specificity	Detects human FGF basic/FGF2/bFGF in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) FGF acidic and rhFGF-7 is observed under non-reducing conditions.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	FGF2
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Dilutions	Immunohistochemistry 5-15 ug/mL Western Blot 0.1 ug/mL
Readout System	Streptavidin Native Protein NBP2-29367 Streptavidin Native Protein NBP2-29370 Streptavidin Native Protein NBP2-29371

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

Gene Symbol	FGF2
Uniprot	P09038()