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FGF basic/FGF2/bFGF Antibody (8W6M5)

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Western Blot: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367] - Analysis of extracts of SKOV3 cells, using FGF basic/FGF2/bFGF Rabbit mAb (NBP3-15367) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367] - Immunofluorescence analysis of HeLa cells using FGF basic/FGF2/bFGF Rabbit mAb (NBP3-15367) at dilution of 1:100 (40x lens). ...read more
Immunohistochemistry-Paraffin: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367] - Mouse brain using [KO Validated] FGF basic/FGF2/bFGF Rabbit mAb (NBP3-15367) at dilution of 1:100 (40x lens).Perform microwave antigen ...read more
Immunohistochemistry-Paraffin: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367] - Rat brain using [KO Validated] FGF basic/FGF2/bFGF Rabbit mAb (NBP3-15367) at dilution of 1:100 (40x lens).Perform microwave antigen ...read more
Immunohistochemistry-Paraffin: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367] - Human colon using [KO Validated] FGF basic/FGF2/bFGF Rabbit mAb (NBP3-15367) at dilution of 1:100 (40x lens).Perform microwave antigen ...read more
Genetic Strategies: Knockout Validated: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367] - Analysis of extracts from normal (control) and FGF basic/FGF2/bFGF knockout (KO) HeLa cells, using FGF basic/FGF2/bFGF ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KO
Clone
8W6M5
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

FGF basic/FGF2/bFGF Antibody (8W6M5) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human FGF basic/FGF2/bFGF (NP_001997.5). RAPERVGGRGRGRGTAAPRAAPAARGSRPGPAGTMAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAE
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
FGF2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Knockout Validated
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for FGF basic/FGF2/bFGF Antibody (8W6M5)

  • basic fibroblast growth factor bFGF
  • Basic fibroblast growth factor
  • bFGF
  • FGF basic
  • FGF2
  • FGF-2
  • FGFBprostatropin
  • fibroblast growth factor 2 (basic)
  • HBGF-2
  • heparin-binding growth factor 2
  • Prostatropin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FGF basic/FGF2/bFGF Antibody (NBP3-15367). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
    • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have several antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we
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Bioinformatics

Gene Symbol FGF2