![Western Blot: Eukaryotic translation initiation factor 5B Antibody [H00009669-B01P] - Analysis of EIF5B expression in human placenta.](http://images.novusbio.com/fullsize/Eukaryotic-translation-initiation-factor-5B-Antibody-Western-Blot-H00009669-B01P-img0006.jpg "Western Blot: Eukaryotic translation initiation factor 5B Antibody [H00009669-B01P] - Analysis of EIF5B expression in human placenta.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | EIF5B (AAH32639.1, 1 a.a. - 1220 a.a.) full-length human protein. MGKKQKNKSEDSTKDDIDLDALAAEIEGAGAAKEQEPQKSKGKKKKEKKKQDFDEDDILKELEELSLEAQGIKADRETVAVKPTENNEEEFTSKDKKKKGQKGKKQSFDDNDSEELEDKDSKSKKTAKPKVEMYSGSDDDDDFNKLPKKAKGKAQKSNKKWDGSEEDEDNSKKIKERSRINSSGESGDESDEFLQSRKGQKKNQKNKPGPNIESGNEDDDASFKIKTVAQKKAEKKERERKKRDEEKAKLRKLKEKEELETGKKDQSKQKESQRKFEEETVKSKVTVDTGVIPASEEKAETPTAAEDDNEGDKKKKDKKKKKGEKEEKEKEKKKGPSKATVKAMQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEATLKLLQAQGVEVPSKDSLPKKRPIYEDKKRKKIPQQLESKEVSESMELCAAVEVMEQGVPEKEETPPPVEPEEEEDTEDAGLDDWEAMASDEETEKVEGNTVHIEVKENPEEEEEEEEEEEEDEESEEEEEEEGESEGSEGDEEDEKVSDEKDSGKTLDKKPSKEMSSDSEYDSDDDRTKEERAYDKAKRRIEKRRLEHSKNVNTEKLRAPIICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKNTKDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEIPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVNLAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKIKILPQYIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEINHKQVDVAKKGQEVCVKIEPIPGESPKMFGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLIVELKKVFEII |
| Specificity | EIF5B - eukaryotic translation initiation factor 5B, |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Mouse |
| Gene | EIF5B |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4) |
| Preservative | No Preservative |
| Purity | Protein A purified |
| Publication using H00009669-B01P | Applications | Species |
|---|---|---|
| Ma HY, Lu CY, Tsao KC et al. Association of EV71 3C polymorphisms with clinical severity. J Microbiol Immunol Infect 2017-06-28 [PMID: 28711442] |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | EIF5B |
| Entrez |
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| Uniprot |
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![Immunocytochemistry/Immunofluorescence: Eukaryotic translation initiation factor 5B Antibody [H00009669-B01P] - Analysis of purified antibody to EIF5B on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/Eukaryotic-translation-initiation-factor-5B-Antibody-Immunocytochemistry-Immunofluorescence-H00009669-B01P-img0004.jpg "Immunocytochemistry/Immunofluorescence: Eukaryotic translation initiation factor 5B Antibody [H00009669-B01P] - Analysis of purified antibody to EIF5B on HeLa cell. (antibody concentration 10 ug/ml)")
![Western Blot: Eukaryotic translation initiation factor 5B Antibody [H00009669-B01P] - Analysis of EIF5B expression in Jurkat.](http://images.novusbio.com/fullsize/Eukaryotic-translation-initiation-factor-5B-Antibody-Western-Blot-H00009669-B01P-img0005.jpg "Western Blot: Eukaryotic translation initiation factor 5B Antibody [H00009669-B01P] - Analysis of EIF5B expression in Jurkat.")
![Western Blot: Eukaryotic translation initiation factor 5B Antibody [H00009669-B01P] - Analysis of EIF5B expression in transfected 293T cell line by EIF5B polyclonal antibody. Lane 1: EIF5B transfected lysate(134.2 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Eukaryotic-translation-initiation-factor-5B-Antibody-Western-Blot-H00009669-B01P-img0007.jpg "Western Blot: Eukaryotic translation initiation factor 5B Antibody [H00009669-B01P] - Analysis of EIF5B expression in transfected 293T cell line by EIF5B polyclonal antibody. Lane 1: EIF5B transfected lysate(134.2 KDa). Lane 2: Non-transfected lysate.")
![Western Blot eIF4E [p Ser209] Antibody](https://images.novusbio.com/images/eIF4E-[p-Ser209]-Antibody-Western-Blot-NB100-81898-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence eIF4E [p Ser209] Antibody](https://images.novusbio.com/images/eIF4E-[p-Ser209]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-81898-img0005.jpg)
![Immunohistochemistry-Paraffin eIF4E [p Ser209] Antibody](https://images.novusbio.com/images/eIF4E-[p-Ser209]-Antibody-Immunohistochemistry-NB100-81898-img0006.jpg)
![Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]](https://images.novusbio.com/images/Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP1-97019-img0004.jpg "Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]")