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EID1 Antibody

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Immunocytochemistry/Immunofluorescence: EID1 Antibody [NBP2-47355] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunocytochemistry/ Immunofluorescence: EID1 Antibody [NBP2-47355] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: EID1 Antibody [NBP2-47355] - Staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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EID1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: MSEMAELSELYEESSDLQMDVMPGEGDLPQMEVGSGSRELSLRPSRSGAQQLEEEGPMEEEEAQPMAAPEGKRSLANGPNAGEQPGQVAGADFESED
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EID1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
EID1 Protein (NBP2-47355PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for EID1 Antibody

  • 21 kDa pRb-associated protein
  • C15orf3PTD014
  • CREBBP/EP300 inhibitor 1
  • CREBBP/EP300 inhibitory protein 1MGC138884
  • CRI1EP300-interacting inhibitor of differentiation 1
  • E1A-like inhibitor of differentiation 1
  • EID-1MGC138883
  • EP300 interacting inhibitor of differentiation 1
  • IRO45620
  • NB4 apoptosis related protein
  • Rb- and p300-binding protein EID-1
  • RBP21
  • retinoblastoma protein-associated protein

Background

Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EID1