![Western Blot: Dynamin [p Ser774] Antibody [NB300-209] - Rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser774 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser774 dynamin. The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/Dynamin-[p-Ser774]-Antibody-Western-Blot-NB300-209-img0002.jpg "Western Blot: Dynamin [p Ser774] Antibody [NB300-209] - Rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser774 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser774 dynamin. The immunolabeling is completely eliminated by treatment with lambda-Ptase.")
| Reactivity | Mu, Rt, Hu, Mu, Bv, Ca, Ch, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Sheep |
| Conjugate | Unconjugated |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 conjugated to KLH. Accession # P21575 |
| Modification | p Ser774 |
| Localization | Cytoplasmic |
| Specificity | Specific for endogenous levels of the ~95 kDa dynamin protein phosphorylated at Ser774. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is blocked by lambda-phosphatase treatment. |
| Predicted Species | Human (100%), Mouse (100%), Bovine (100%), Canine (100%), Chicken (100%), Primate (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Sheep |
| Gene | DNM1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | NB300-209 can be used in Western blot where a band is seen at ~ 97 kD, representing Dynamin |
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| Theoretical MW | 95 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Preservative | No Preservative |
| Purity | Antigen Affinity-purified |
| Publication using NB300-209 | Applications | Species |
|---|---|---|
| Tan, TC et al. Cdk5 is Essential for Synaptic Vesicle Endocytosis. Nat. Cell Bio., 5(8): 701-710. 2003-01-01 [PMID: 12855954] |
Secondary Antibodies |
Isotype Controls |
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