![Western Blot: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Western blot analysis of DPPIV/CD26 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse lung tissue lysate Lane 2: mouse heart tissue lysate Lane 3: human kidney tissue lysate Lane 4: Raji cell lysate Lane 5: PC-3 cell lysate](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-JM11-42-Western-Blot-NBP2-78791-img0007.jpg "Western Blot: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Western blot analysis of DPPIV/CD26 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse lung tissue lysate Lane 2: mouse heart tissue lysate Lane 3: human kidney tissue lysate Lane 4: Raji cell lysate Lane 5: PC-3 cell lysate")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, KO |
| Clone | JM11-42 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Synthetic peptide within Human DPPIV/CD26 aa 701-747 / 766 (Extracellular). (SwissProt: P27487 Human; SwissProt: P28843 Mouse; SwissProt: P14740 Rat) |
| Localization | Secreted, Cell membrane, Apical cell membrane, invadopodium membrane, lamellipodium membrane, Cell junction, Membrane raft. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | DPP4 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for DPPIV/CD26 Antibody (NBP2-78791)Find related products by research area.
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| Gene Symbol | DPP4 |
![Immunocytochemistry/Immunofluorescence: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Staining CD26 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-JM11-42-Immunocytochemistry-Immunofluorescence-NBP2-78791-img0005.jpg "Immunocytochemistry/Immunofluorescence: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Staining CD26 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Analysis of paraffin-embedded human liver tissue using anti-CD26 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-JM11-42-Immunohistochemistry-Paraffin-NBP2-78791-img0006.jpg "Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Analysis of paraffin-embedded human liver tissue using anti-CD26 antibody. Counter stained with hematoxylin.")
![Flow Cytometry: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Analysis of Hela cells with CD26 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-JM11-42-Flow-Cytometry-NBP2-78791-img0004.jpg "Flow Cytometry: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Analysis of Hela cells with CD26 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.")
![Immunocytochemistry/Immunofluorescence: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Staining CD26 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-JM11-42-Immunocytochemistry-Immunofluorescence-NBP2-78791-img0002.jpg "Immunocytochemistry/Immunofluorescence: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Staining CD26 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Analysis of paraffin-embedded rat epididymis tissue using anti-CD26 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-JM11-42-Immunohistochemistry-Paraffin-NBP2-78791-img0003.jpg "Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Analysis of paraffin-embedded rat epididymis tissue using anti-CD26 antibody. Counter stained with hematoxylin.")
![Knockout Validated: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - All lanes: Western blot analysis of CD26 with anti-CD26 antibody at 1:500 dilution.Lane 1: Wild-type Hela whole cell lysate (10 ug). Lane 2: CD26 knockout Hela whole cell lysate (10 ug).NBP2-78791 was shown to specifically react with CD26 in wild-type Hela cells. NO band was observed when CD26 knockout sample was tested. Wild-type and CD26 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-JM11-42-Knockout-Validated-NBP2-78791-img0009.jpg "Knockout Validated: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - All lanes: Western blot analysis of CD26 with anti-CD26 antibody at 1:500 dilution.Lane 1: Wild-type Hela whole cell lysate (10 ug). Lane 2: CD26 knockout Hela whole cell lysate (10 ug).NBP2-78791 was shown to specifically react with CD26 in wild-type Hela cells. NO band was observed when CD26 knockout sample was tested. Wild-type and CD26 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")