DNMT3A Antibody

Methylation of cytosine residues is essential for mammalian development by regulating gene expression, gene silencing and genomic imprinting (1). This process is controlled by the enzyme DNA (cytosine-5)-methyltransferase 3A (DNMt3a), which is encoded by the DNMT3A gene and has a predicted molecular weight of 102 kDa. DNA methyltransferases including DNMt3a are involved in de novo cytosine methylation and maintenance of embryonic and somatic cells through the transfer of methyl groups to specific CpG structures in DNA (2). Somatic mutations in DNMT3A are responsible for cytogenetically normal acute myeloid leukemia (CN-AML). Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA are linked with cancer (3,4). Mutations in the DNMT3A gene have also been found to cause DNMT3A overgrowth syndrome and myelodysplastic syndromes.

References

1. Thakur, A., Mackin, S. J., Irwin, R. E., O'Neill, K. M., Pollin, G., & Walsh, C. (2016). Widespread recovery of methylation at gametic imprints in hypomethylated mouse stem cells following rescue with DNMT3A2. Epigenetics Chromatin, 9, 53. doi:10.1186/s13072-016-0104-2

2. Ravichandran, M., Lei, R., Tang, Q., Zhao, Y., Lee, J., Ma, L., . . . Dawlaty, M. M. (2019). Rinf Regulates Pluripotency Network Genes and Tet Enzymes in Embryonic Stem Cells. Cell Rep, 28(8), 1993-2003.e1995. doi:10.1016/j.celrep.2019.07.080

3. Pang, Y., Liu, J., Li, X., Xiao, G., Wang, H., Yang, G., . . . Ren, H. (2018). MYC and DNMT3A-mediated DNA methylation represses microRNA-200b in triple negative breast cancer. J Cell Mol Med, 22(12), 6262-6274. doi:10.1111/jcmm.13916

4. Xia, L., Huang, W., Bellani, M., Seidman, M. M., Wu, K., Fan, D., . . . Baylin, S. B. (2017). CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes. Cancer Cell, 31(5), 653-668.e657. doi:10.1016/j.ccell.2017.04.005

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.

Showing Publications 1 - 10 of 12. Show All 12 Publications. Collapse Publications.

Publications using NB100-265	Applications	Species
Xie Z, Wang Q, Hu S. Coordination of PRKCA/PRKCA-AS1 interplay facilitates DNA methyltransferase 1 recruitment on DNA methylation to affect protein kinase C alpha transcription in mitral valve of rheumatic heart disease Bioengineered 2021-09-06 [PMID: 34482802]
Yang J, Wu R, Li W et al. The triterpenoid corosolic acid blocks transformation and epigenetically reactivates Nrf2 in TRAMP-C1 prostate cells. Mol. Carcinog. 2017-12-16 [PMID: 29247555] (Mouse)		Mouse
Selvakumar T, Gjidoda A, Hovde SL, Henry RW. Regulation of human RNA polymerase III transcription by DNMT1 and DNMT3a DNA methyltransferases. J Biol Chem. 2012-03-02 [PMID: 22219193] (WB, Human)	WB	Human
Kaminskyy V, Surova O, Vaculova A, Zhivotovsky B. Combined inhibition of DNA methyltransferase and histone deacetylase restores caspase-8 expression and sensitizes SCLC cells to TRAIL. Carcinogenesis. 2011-07-18 [PMID: 21771726]
Al-Salihi M, Yu M, Burnett DM et al. The depletion of DNA methyltransferase-1 and the epigenetic effects of 5-aza-2'deoxycytidine (decitabine) are differentially regulated by cell cycle progression. Epigenetics 2011-08-01 [PMID: 21725200] (Human)		Human
Shikauchi Y et al. SALL3 interacts with DNMT3A and shows the ability to inhibit CpG island methylation in hepatocellular carcinoma. Mol Cell Biol;29(7):1944-58. 2009-04-01 [PMID: 19139273]
Fanelli M, Caprodossi S, Ricci-Vitiani L et al. Loss of pericentromeric DNA methylation pattern in human glioblastoma is associated with altered DNA methyltransferases expression and involves the stem cell compartment. Oncogene 2008-01-10 [PMID: 17653095] (Human)		Human
Reynolds PA et al. Tumor suppressor p16INK4A regulates polycomb-mediated DNA hypermethylation in human mammary epithelial cells. J Biol Chem;281(34):24790-802. 2006-08-25 [PMID: 16766534]
La Salle S, Trasler JM. Dynamic expression of DNMT3a and DNMT3b isoforms during male germ cell development in the mouse. Dev Biol 2006-08-01 [PMID: 16725135] (Mouse)		Mouse
Robert MF, Morin S, Beaulieu N et al. DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells. Nat Genet;33(1):61-5. 2003-01-01 [PMID: 12496760] (WB, Human)	WB	Human
Weisenberger DJ, Velicescu M, Preciado-Lopez MA et al. Identification and characterization of alternatively spliced variants of DNA methyltransferase 3a in mammalian cells. Gene;298(1):91-9. 2002-09-18 [PMID: 12406579] (Human, Mouse)		Human, Mouse
Beaulieu N et al. An essential role for DNA methyltransferase DNMT3B in cancer cell survival. J Biol Chem;277(31):28176-81. 2002-08-02 [PMID: 12015329] (WB, Human)	WB	Human
Show All 12 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Verified Customer	ICC	Human	11/18/2017	View Summary Application Immunocytochemistry Sample Tested fibroblasts Species Human Lot c-3 Comments Comments Diluted 1:50 in 1X PBS/1% BSA/0.3% Triton™ X-100, 16 hours at 4C incubation. 2 hours (25C) secondary (1.5:1000 dilution)

Array NB100-265

• DNMT3A Antibodies
• DNMT3A ELISA Kits
• DNMT3A Kit
• DNMT3A Lysate
• DNMT3A Proteins