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DMC1 Antibody (2H12/4) [Janelia Fluor® 635]

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Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP
Clone
2H12/4
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 635

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-2617AF350
Alexa Fluor 405
NB100-2617AF405
Alexa Fluor 488
NB100-2617AF488
Alexa Fluor 532
NB100-2617AF532
Alexa Fluor 594
NB100-2617AF594
Alexa Fluor 647
NB100-2617AF647
Alexa Fluor 700
NB100-2617AF700
Alexa Fluor 750
NB100-2617AF750
Biotin
NB100-2617B
CoraFluor 1
NB100-2617CL1
DyLight 350
NB100-2617UV
DyLight 405
NB100-2617V
DyLight 488
NB100-2617G
DyLight 550
NB100-2617R
DyLight 594
NB100-2617DL594
DyLight 650
NB100-2617C
DyLight 680
NB100-2617FR
DyLight 755
NB100-2617IR
FITC
NB100-2617F
HRP
NB100-2617H
Janelia Fluor 525
NB100-2617JF525
Janelia Fluor 549
NB100-2617JF549
Janelia Fluor 585
NB100-2617JF585
Janelia Fluor 646
NB100-2617JF646
Janelia Fluor 669
NB100-2617JF669
Unconjugated
NB100-2617
mFluor Violet 450 SE
NB100-2617MFV450
mFluor Violet 500 SE
NB100-2617MFV500
mFluor Violet 610 SE
NB100-2617MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-2617
BSA Free
NB100-2617
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DMC1 Antibody (2H12/4) [Janelia Fluor® 635] Summary

Immunogen
Human DMC1 protein (expressed as 6xHis fusion in E. coli and band cut out of gel of insoluble pelet)
Localization
Nuclear
Specificity
Specific for DMC1 - does not cross-react with the related protein Rad51.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
DMC1
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for DMC1 Antibody (2H12/4) [Janelia Fluor® 635]

  • disrupted meiotic cDNA1, yeast, homolog of
  • DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologousrecombination (yeast)
  • DMC1 homologue
  • HsLim15
  • LIM15yeast homolog) meiosis-specific homologousrecombination
  • meiotic recombination protein DMC1/LIM15 homolog
  • MGC150472
  • MGC150473

Background

DMC-1 is a meiosis-specific homologue of RecA/Rad51 and is an essential component of the meiotic recombination machinery in yeast and higher eukaryotes. DMC-1 is the human equivalent of e. coli recA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Check out the latest blog posts on DMC1.
Determining DMC1's role in Homologus Recombination
The DMC1 gene encodes a 36.7 kDa nuclear protein involved in meiotic homologous recombination. This recombinase is functionally related to the yeast RAD51 and E. coli RecA genes. In contrast to RAD51, which functions in both mitotic and meiotic recomb...  Read full blog post.
mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol DMC1