![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of 1 month old zebrafish embryo](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunocytochemistry-Immunofluorescence-NBP1-97715-img0006.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of 1 month old zebrafish embryo")
| Reactivity | Hu, Mu, Rt, Po, Ca, Ch, Gp, Ha, Rb, ZeSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | RGE53 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product. |
| Immunogen | Derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal preparation of HeLa cells. |
| Marker | Epithelial Marker |
| Specificity | This antibody reacts exclusively with Cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT18 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is suitable for immunoblotting, immunocytochemistry on acetone fixed cells, immunohistochemistry on frozen sections and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications. This antibody is CyTOF ready. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, pH 7.2 |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 18 Antibody (NBP1-97715)Find related products by research area.
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Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | KRT18 |
![Immunohistochemistry: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Immunofluorescence staining of human colon epithelium.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunohistochemistry-NBP1-97715-img0007.jpg "Immunohistochemistry: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Immunofluorescence staining of human colon epithelium.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of a 2 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunocytochemistry-Immunofluorescence-NBP1-97715-img0004.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of a 2 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of a 5 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunocytochemistry-Immunofluorescence-NBP1-97715-img0005.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of a 5 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining")
![Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Frozen section of human colon](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunohistochemistry-Frozen-NBP1-97715-img0002.jpg "Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Frozen section of human colon")
![Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Frozen section of human kidney epithelium](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunohistochemistry-Frozen-NBP1-97715-img0001.jpg "Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Frozen section of human kidney epithelium")
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