Coronin-1a Antibody

Highly conserved coronin family of proteins/actin regulators from WD-repeat superfamily were originally identified in Dictyostelium, wherein, coronin deletions were observed to affect actin-dependent processes, such as phagocytosis, cell motility, and cytokinesis. Coronin-1a (Coro1A) belongs to Type I coronins, almost exclusively expressed in hematopoietic lineages, and plays a key role during T lymphocyte activation, migration, survival, and calcium signaling. Moreover, Coronin-1a has been documented to be dispensable for actin-dependent functions in macrophages. Coro1a-deficient mice have reduced peripheral T cells due to both an impaired survival of naive cells and migrational defects. Both CD4+ and CD8+ T cell subsets are decreased in the periphery of Coro1a-/- mice while thymic cellularity and subpopulations are similar to those observed in wild-type mice. A nonsense mutation in Coro1a has been shown to protect against systemic lupus in a mouse model. Moreover, it has shown that Coro1a is mutated in a thymic egress-deficient mouse strain, but also in a patient with severe combined immunodeficiency (SCID).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: ICC/IF, WB, Western Blot.

Showing Publications 1 - 4 of 4.

Publications using NB110-58867	Applications	Species
Williams A, Khatkar P, Branscome H et al. The Use of CBD and Its Synthetic Analog HU308 in HIV-1-Infected Myeloid Cells Pharmaceuticals (Basel) 2023-08-12 [PMID: 37631062] (Western Blot)	Western Blot
Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods 2018-01-11 [PMID: 30377371] (Human) Details: Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).		Human
Tweedell R, Tao D, Dinglasan RR. The cellular and proteomic response of primary and immortalized murine Kupffer cells following immune stimulation diverges from that of monocyte-derived macrophages. Proteomics. 2014-09-30 [PMID: 25266554] (ICC/IF, WB, Rat) Details: Coronin-1a antibody used for WB at 1:5000 dilution on lysates of RKC1/rat Kupffer cell line exposed or not to LPS (Figure 2A). Antibody also used for ICC-IF at 1:500 dilution on primary rat Kupffer cells and RKC1 cells with or without LPS stimulation.	ICC/IF, WB	Rat
Stray-Pedersen A, Jouanguy E, Crequer A et al. Compound Heterozygous CORO1A Mutations in Siblings with a Mucocutaneous-Immunodeficiency Syndrome of Epidermodysplasia Verruciformis-HPV, Molluscum Contagiosum and Granulomatous Tuberculoid Leprosy. J. Clin. Immunol. 2014-07-30 [PMID: 25073507] (WB, Human) Details: Coronin-1a antibody used in WB for detecting CORO1A clinical phenotype/ deficiency of coronin-1A protein in study involving siblings with a mucocutaneous-immunodeficiency syndrome of spidermodysplasia verruciformis-HPV, molluscum contagiosum and granulomatous tuberculoid leprosy. Lysates of lymphoblastoid/lymphoid cell lines from P2 (affected sibling) and P3/P4 (parental carriers) were prepared in 1 % NP-40 lysis buffer/HALT protease inhibitor, blot blocked with 3% skim milk followed by primary incubation and detection with Odyssey/Licor method (Fig 6 - Coronin-1a band size 57kDa; Loading control used Myosin IIA).	WB	Human

Array NB110-58867

• Coronin-1a Antibodies
• Coronin-1a ELISA Kits
• Coronin-1a Lysate
• Coronin-1a Proteins