Home » Coagulation Factor II/Thrombin » Thrombin ELISA Kits » Porcine Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)
Novus Biologicals products are now on bio-techne.com

Porcine Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

 (1 Publication)
 See All Related
Submit a Review

Images

 
(
Enlarge)
ELISA: Coagulation Factor II/Thrombin ELISA Kit [Biotin] [KA1407] - The curve is provided for illustration only. A standard curve should be generated each time the assay is performed.
ELISA: Coagulation Factor II/Thrombin ELISA Kit [Biotin] [KA1407] - The curve is provided for illustration only. A standard curve should be generated each time the assay is performed.

Product Details

Summary
Reactivity PoSpecies Glossary
Applications ELISA, NULL
Suitable Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum
Standard Curve Range
0.391 to 100 ng/mL
Sensitivity
0.24 ng/mL

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Porcine Coagulation Factor II/Thrombin ELISA Kit (Colorimetric) Summary

Description
F2 (Pig) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of pig F2.
Standard Curve Range
0.391 to 100 ng/mL
Sensitivity
0.24 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 7.4%
Intra-Assay
CV% < 4.8%
Spike Recovery
96%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
F2

Applications/Dilutions

Dilutions
  • ELISA
  • Quantification
Publications
Read Publication using
KA1407 in the following applications:

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Swine Prothrombin Antibody (100x): A 100-fold concentratedbiotinylated polyclonal antibody against swine prothrombin.
  2. Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substratetetramethylbenzidine.
  3. EIA Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
  4. Sealing Tapes: Pressure-sensitive sealing tapes that can be cut to fit the format ofthe individual assay.
  5. Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction.
  6. Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate.
  7. Swine Prothrombin Microplate: A 96-well polystyrene microplate coated with amonoclonal antibody against swine prothrombin.
  8. Swine Prothrombin Standard: Swine prothrombin in a buffered protein base,lyophilized.
  9. Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Porcine Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

  • coagulation factor II (thrombin) receptor-like 2
  • Coagulation factor II receptor-like 2 (protease-actovated receptor 3)
  • Coagulation factor II receptor-like 2
  • Coagulation Factor II
  • F2
  • PAR-3
  • PAR3proteinase-activated receptor 3
  • protease-activated receptor 3
  • proteinase-activated receptor-3
  • PT
  • serine protease
  • Thrombin receptor-like 2
  • Thrombin

Background

Proteinase-activated receptor 3 (PAR3) is a member of the Proteinase-Activated Receptor subfamily. The enzyme thrombin is involved in the activation of platelets, leukocytes, endothelial cells, and mesenchymal cells at sites of vascular injury. These cellular responses are triggered through proteolytic activation of PAR3 and other receptors by thrombin. It is believed that PAR3 functions as a cofactor for the cleavage and activation of PAR4. PAR3 expression has been documented in blood, bone marrow, lung, spleen, and vessel. ESTs have been isolated from head/neck, skeletal muscle, and skin libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Coagulation Factor
III/Tissue Factor [HR ...
DCF300
N/A Coagulation Factor III/Tissue Factor [HRP]
N/A Coagulation Factor III/Tissue Factor [HRP]
(25 Publications)
Species: Hu
Applications: ELISA
Serpin
C1/Antithrombin-III A ...
AF1267
Serpin C1/Antithrombin-III Antibody [Unconjugated]
(5 Publications)
Species: Hu
Applications: IP, Neut, WB
Recombinant Human
PRH2 GST (N-Term) Pro ...
H00005555-P01
SDS-PAGE Recombinant Human PRH2 GST (N-Term) Protein
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
Factor V Antibody
(HL2421) - Azide and ...
NBP3-25459
Western Blot Factor V Antibody (HL2421) - Azide and BSA Free
Western Blot Factor V Antibody (HL2421) - Azide and BSA Free
Species: Hu, Rt
Applications: WB
Coagulation Factor
X Antibody
NBP1-33320
Western Blot Coagulation Factor X Antibody
Immunocytochemistry/ Immunofluorescence Coagulation Factor X Antibody
Immunohistochemistry-Paraffin Coagulation Factor X Antibody
(2 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
Albumin Antibody
(188835) [Unconjugate ...
MAB1455
Simple Western Albumin Antibody (188835) [Unconjugated] - Serum
Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum
Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum
(3 Reviews)
(54 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
Factor VIII
Antibody - BSA Free
NB100-91761
Western Blot Factor VIII Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence Factor VIII Antibody - BSA Free
Western Blot Factor VIII Antibody - BSA Free
(5 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
DBT Antibody
NBP1-89522
Immunocytochemistry/ Immunofluorescence DBT Antibody
Immunohistochemistry-Paraffin DBT Antibody
Immunohistochemistry-Paraffin DBT Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Contactin-1
Antibody [Unconjugate
AF904
Western Blot Contactin-1 Antibody [Unconjugated]
Immunohistochemistry Contactin-1 Antibody [Unconjugated]
Neutralization Contactin-1 Antibody [Unconjugated]
(21 Publications)
Species: Hu, Mu, Rt
Applications: IHC, Neut, Simple Western, WB
Plasminogen
Antibody
NB600-930
Western Blot Plasminogen Antibody
Western Blot Plasminogen Antibody
Western Blot Plasminogen Antibody
(2 Reviews)
(6 Publications)
Species: Hu, Rt
Applications: ELISA, WB
MTHFR Antibody
(5D3) - BSA Free
NBP2-37607
Immunohistochemistry-Paraffin MTHFR Antibody (5D3) - BSA Free
ELISA MTHFR Antibody (5D3) - BSA Free
Western Blot MTHFR Antibody (5D3) - BSA Free
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, WB
Serpin E1/PAI-1
Antibody - BSA Free
NBP1-19773
Western Blot Serpin E1/PAI-1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence Serpin E1/PAI-1 Antibody - BSA Free
Immunohistochemistry-Paraffin Serpin E1/PAI-1 Antibody - BSA Free
(2 Reviews)
(32 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
Von Willebrand
Factor Antibody
NB600-586
Dual RNAscope ISH-IHC Von Willebrand Factor Antibody
Immunohistochemistry-Frozen Von Willebrand Factor Antibody
Immunohistochemistry Von Willebrand Factor Antibody
(3 Reviews)
(14 Publications)
Species: Ca, Fe, Hu, Mu
Applications: Dual ISH-IHC, ICC/IF, IHC, IHC-Fr, IHC-P
TAT Antibody
NBP1-79286
Western Blot TAT Antibody
(1 Review)
Species: Rt
Applications: WB
ATAT1 Antibody
NBP2-48990
Immunocytochemistry/ Immunofluorescence ATAT1 Antibody
Immunohistochemistry-Paraffin ATAT1 Antibody
Immunohistochemistry-Paraffin ATAT1 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Sialin/SLC17A5
Antibody
NBP2-20383
Immunohistochemistry-Paraffin Sialin/SLC17A5 Antibody
Western Blot Sialin/SLC17A5 Antibody
Immunocytochemistry/ Immunofluorescence Sialin/SLC17A5 Antibody
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
t-Plasminogen
Activator/tPA [HRP]
DTPA00
N/A t-Plasminogen Activator/tPA [HRP]
N/A t-Plasminogen Activator/tPA [HRP]
(7 Publications)
Species: Hu
Applications: ELISA
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(807 Publications)
Species: Hu
Applications: BA

Publications for Coagulation Factor II/Thrombin ELISA Kit (KA1407)(1)

We have publications tested in 1 confirmed species: Porcine.

We have publications tested in 1 application: ELISA.


Filter By Application
ELISA
(1)
All Applications
Filter By Species
Porcine
(1)
All Species

Reviews for Coagulation Factor II/Thrombin ELISA Kit (KA1407) (0)

There are no reviews for Coagulation Factor II/Thrombin ELISA Kit (KA1407). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Coagulation Factor II/Thrombin ELISA Kit (KA1407). (Showing 1 - 1 of 1 FAQ).

  1. Do you have neutralizing antibodies for anti-mouse Thrombin?
    • We currently have 1 neutralizing Thrombin antibody (NBP1-95029), however it is an anti-human antibody and has only been validated in human samples. If you would like to test this antibody in mouse samples, you may be interested in participating in our Innovators Reward Program.

Additional Coagulation Factor II/Thrombin Products

Research Areas for Coagulation Factor II/Thrombin ELISA Kit (KA1407)

Find related products by research area.

Blogs on Coagulation Factor II/Thrombin

There are no specific blogs for Coagulation Factor II/Thrombin, but you can read our latest blog posts.

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005
Immunohistochemistry-Paraffin: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - Human Placenta Tissue
Coagulation Factor II/Thrombin Antibody
NBP1-58268
Simple Western lane view shows lysates of human serum, loaded at 1:25000. A specific band was detected for Albumin at approximately 64&nbsp;kDa (as indicated) using 1&nbsp;µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) . This experiment was conducted under reducing&nbsp;conditions and using the 12-230 kDa separation system.
Albumin Antibody (188835) [Unconjugated] - Serum
MAB1455

Contact Information

Product PDFs

  F2 (Pig) ELISA Kit
  PDF Datasheet
  Request CofA

Review this Product

Be the first to review our Porcine Coagulation Factor II/Thrombin ELISA Kit (Colorimetric) and receive a gift card or discount.


Bioinformatics

Gene Symbol F2