![Immunohistochemistry-Paraffin: CFTR Antibody (CFTR/1643) - Azide and BSA Free [NBP2-54509] - Formalin-fixed, paraffin-embedded Human Pancreas stained with CFTR Monoclonal Antibody (CFTR/1643).](http://images.novusbio.com/fullsize/CFTR-Antibody-CFTR-1643-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54509-img0001.jpg "Immunohistochemistry-Paraffin: CFTR Antibody (CFTR/1643) - Azide and BSA Free [NBP2-54509] - Formalin-fixed, paraffin-embedded Human Pancreas stained with CFTR Monoclonal Antibody (CFTR/1643).")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, IHC, MA |
| Clone | CFTR/1643 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53375). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Recombinant fragment (around aa 258-385) of human CFTR protein (exact sequence is proprietary) (Uniprot: P13569) |
| Localization | Cell Surface and Cytoplasmic |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CFTR |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 167 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for CFTR Antibody (NBP2-54509)Find related products by research area.
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The ABCA1 Antibody and ABC Transporter Research The ABC (ATP-binding cassette) transporters are a superfamily of transmembrane proteins which play an essential role in membrane transport in both prokaryotes and eukaryotes. In humans, ABC transporters have been implicated in many conditions. For exa... Read full blog post. |
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| Gene Symbol | CFTR |
![Flow Cytometry: CFTR Antibody (CFTR/1643) - Azide and BSA Free [NBP2-54509] - Analysis of MOLT-4 cells using CFTR Antibody (CFTR/1643) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CFTR-Antibody-CFTR-1643-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54509-img0002.jpg "Flow Cytometry: CFTR Antibody (CFTR/1643) - Azide and BSA Free [NBP2-54509] - Analysis of MOLT-4 cells using CFTR Antibody (CFTR/1643) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).")
![Protein Array: CFTR Antibody (CFTR/1643) - Azide and BSA Free [NBP2-54509] - Analysis of Protein Array containing >19,000 full-length human proteins using CFTR Antibody (CFTR/1643) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/CFTR-Antibody-CFTR-1643-Azide-and-BSA-Free-Protein-Array-NBP2-54509-img0003.jpg "Protein Array: CFTR Antibody (CFTR/1643) - Azide and BSA Free [NBP2-54509] - Analysis of Protein Array containing >19,000 full-length human proteins using CFTR Antibody (CFTR/1643) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.")
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