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CD117/c-kit Antibody (2B8) - Azide and BSA Free

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Flow Cytometry: CD117/c-kit Antibody (2B8) - Azide and BSA Free [NBP2-80624] - Detection of CD117/c-kit in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with PE-conjugated Rat Anti-Mouse B220/CD45R (Catalog # FAB1217P) and Rat Anti-Mouse CD117/c-kit Monoclonal Antibody (Catalog # NB100-77477, top panel) or isotype control antibody (Catalog # MAB0061, bottom panel), followed by APC-conjugated Goat Anti-Rat IgG (Catalog # F0113). Image from the standard format of this antibody.
Flow Cytometry: CD117/c-kit Antibody (2B8) - Azide and BSA Free [NBP2-80624] - Detection of CD117/c-kit in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with PE-conjugated Rat ...read more
Flow Cytometry: CD117/c-kit Antibody (2B8) - Azide and BSA Free [NBP2-80624] - C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC. Image from the standard format of this antibody.
Flow Cytometry: CD117/c-kit Antibody (2B8) - Azide and BSA Free [NBP2-80624] - Identification of CD117/c-kit expressing bone marrow cells.Double labeling of mouse bone marrow cells with anti-CD45 antibody and rabbit ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow, ICC/IF, IHC, IP, CyTOF-ready
Clone
2B8
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-77477AF350
Alexa Fluor 532
NB100-77477AF532
Alexa Fluor 594
NB100-77477AF594
Alexa Fluor 750
NB100-77477AF750
Allophycocyanin
NB100-77477APC-0.1ml
Allophycocyanin/Cy7
NB100-77477APCCY7
Biotin
NB100-77477B
DyLight 350
NB100-77477UV-0.1ml
DyLight 405
NB100-77477V-0.1ml
DyLight 488
NB100-77477G
DyLight 550
NB100-77477R
DyLight 594
NB100-77477DL594
DyLight 650
NB100-77477C
DyLight 680
NB100-77477FR-0.1ml
DyLight 755
NB100-77477IR-0.1ml
FITC
NB100-77477F
HRP
NB100-77477H
Janelia Fluor 549
NB100-77477JF549
Janelia Fluor 646
NB100-77477JF646
PE
NB100-77477PE-0.1ml
PE/Atto594
NB100-77477PEATT594
PE/Cy5.5
NB100-77477PECY55
PE/Cy7
NB100-77477PECY7
PerCP
NB100-77477PCP-0.1ml
Unconjugated
NB100-77477-0.1mg
mFluor Violet 450 SE
NB100-77477MFV450
mFluor Violet 500 SE
NB100-77477MFV500
mFluor Violet 610 SE
NB100-77477MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-77477-0.1mg
BSA Free
NB100-77477SS
BSA Free
NB100-77477-0.1mg
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CD117/c-kit Antibody (2B8) - Azide and BSA Free Summary

Immunogen
Mouse bone marrow mast cells
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
KIT
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.6 ug/10^6 cells in 100 uL
  • Immunocytochemistry/ Immunofluorescence 0.25 ug/10^6 cells in 100 uL. Use reported in scientific literature (PMID 28097227)
  • Immunohistochemistry 1:20 - 1:1000
  • Immunohistochemistry-Frozen 1:20 - 1:1000
  • Immunoprecipitation 1:20 - 1:1000
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23967247).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for CD117/c-kit Antibody (2B8) - Azide and BSA Free

  • CD117 antigen
  • CD117
  • ckit
  • c-kit
  • EC 2.7.10
  • EC 2.7.10.1
  • KIT
  • PBT
  • piebald trait
  • Proto-oncogene c-Kit
  • proto-oncogene tyrosine-protein kinase Kit
  • SCF R
  • SCFR
  • SCFRmast/stem cell growth factor receptor
  • soluble KIT variant 1
  • Tyrosine-protein kinase Kit
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene ...  Read full blog post.
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Bioinformatics

Gene Symbol KIT