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Human Caspase-6 ELISA Kit (Colorimetric)

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ELISA: Human Caspase-6 ELISA Kit (Colorimetric) [NBP2-75028] - Samples were spiked with high concentrations of Human Caspase-6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human Caspase-6 ELISA Kit (Colorimetric) [NBP2-75028] - Samples were spiked with high concentrations of Human Caspase-6 and diluted with Reference Standard & Sample Diluent to produce samples with values within ...read more
ELISA: Human Caspase-6 ELISA Kit (Colorimetric) [NBP2-75028] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL

Order Details

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Human Caspase-6 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human CASP6 in samples. No significant cross-reactivity or interference between Human CASP6 and analogues was observed.
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.61%
Intra-Assay
CV% < 5.43%
Spike Recovery
92-110%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CASP6

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Caspase-6 ELISA Kit (Colorimetric)

  • Apoptotic protease Mch-2
  • CASP6
  • CASP-6
  • caspase 6, apoptosis-related cysteine peptidase
  • caspase 6, apoptosis-related cysteine protease
  • Caspase6
  • Caspase-6
  • EC 3.4.22
  • Mch2
  • MCH2EC 3.4.22.59

Background

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-6 is a major apoptotic executioner (1,2). It exists as a pro-domain protein and is cleaved by caspase-3 into two active subunits, an 18 kDa and a 10 kDa subunit (3). Caspase-6 cleaves among many different intracellular proteins, nuclear lamins. Lamins play a significant role in maintaining the integrity of the cell membrane, and their cleavage results in subsequent cellular breakdown and death (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

  SDS
  Human Caspase-6 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol CASP6