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Caspase-6 Antibody

Images

 
Independent Antibodies: Western Blot: Caspase-6 Antibody [NBP1-87684] - Analysis using Anti-CASP6 antibody NBP1-87684 (A) shows similar pattern to independent antibody NBP1-87683 (B).
Orthogonal Strategies: Immunohistochemistry-Paraffin: Caspase-6 Antibody [NBP1-87684] - Staining in human small intestine and skeletal muscle tissues using anti-CASP6 antibody. Corresponding CASP6 RNA-seq data ...read more
Immunohistochemistry-Paraffin: Caspase-6 Antibody [NBP1-87684] - Staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Caspase-6 Antibody [NBP1-87684] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: Caspase-6 Antibody [NBP1-87684] - Staining of human small intestine shows high expression.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Caspase-6 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MSSASGLRRGHPAGGEENMTETDAFYKREMFDPAEKYKMDHRRRGIALIFNHERFFWHLTLPERRGTCADRDNLTRRF
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control
Jurkat Staurosporine Treated / Untreated Cell Lysate
Control Peptide
Caspase-6 Protein (NBP1-87684PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Caspase-6 Antibody

  • Apoptotic protease Mch-2
  • CASP6
  • CASP-6
  • caspase 6, apoptosis-related cysteine peptidase
  • caspase 6, apoptosis-related cysteine protease
  • Caspase6
  • Caspase-6
  • EC 3.4.22
  • Mch2
  • MCH2EC 3.4.22.59

Background

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-6 is a major apoptotic executioner (1,2). It exists as a pro-domain protein and is cleaved by caspase-3 into two active subunits, an 18 kDa and a 10 kDa subunit (3). Caspase-6 cleaves among many different intracellular proteins, nuclear lamins. Lamins play a significant role in maintaining the integrity of the cell membrane, and their cleavage results in subsequent cellular breakdown and death (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-6 Antibody (NBP1-87684) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Caspase-6 Antibody (NBP1-87684) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Caspase-6.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

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Bioinformatics

Gene Symbol CASP6