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Carbonic Anhydrase I/CA1 Antibody (M1)

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Western Blot: Carbonic Anhydrase I/CA1 Antibody (M1) [H00000759-M05] - Analysis of CA1 expression in transfected 293T cell line by CA1 monoclonal antibody (M05), clone M1. Lane 1: CA1 transfected lysatE (28.9 KDa). Lane 2: Non-transfected lysate.
Western Blot: Carbonic Anhydrase I/CA1 Antibody (M1) [H00000759-M05] - Analysis of CA1 expression in transfected 293T cell line by CA1 monoclonal antibody (M05), clone M1. Lane 1: CA1 transfected lysatE (28.9 KDa). Lane ...read more
Western Blot: Carbonic Anhydrase I/CA1 Antibody (M1) [H00000759-M05] - Analysis of CA1 expression in HeLa (Cat # L013V1).
Western Blot: Carbonic Anhydrase I/CA1 Antibody (M1) [H00000759-M05] - Analysis of CA1 expression in human spleen.
ELISA: Carbonic Anhydrase I/CA1 Antibody (M1) [H00000759-M05] - Detection limit for recombinant GST tagged CA1 is approximately 0.03ng/ml as a capture antibody.
Immunoprecipitation: Carbonic Anhydrase I/CA1 Antibody (M1) [H00000759-M05] - Analysis of CA1 transfected lysate using anti-CA1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CA1 rabbit ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IP
Clone
M1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Carbonic Anhydrase I/CA1 Antibody (M1) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
CA1 (AAH27890, 1 a.a. ~ 261 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASPDWGYDDKNGPEQWSKLYPIANGNNQSPVDIKTSETKHDTSLKPISVSYNPATAKEIINVGHSFHVNFEDNDNRSVLKGGPFSDSYRLFQFHFHWGSTNEHGSEHTVDGVKYSAELHVAHWNSAKYSSLAEAASKADGLAVIGVLMKVGEANPKLQKVLDALQAIKTKGKRAPFTNFDPSTLLPSSLDFWTYPGSLTHPPLYESVTWIICKESISVSSEQLAQFRSLLSNVEGDNAVPMQHNNRPTQPLKGRTVRASF
Specificity
CA1 - carbonic anhydrase I
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CA1
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunoprecipitation
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate, tissue lysate and recombinant protein for WB. It has been used for ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Carbonic Anhydrase I/CA1 Antibody (M1)

  • CA1
  • CA-I
  • Car1
  • Carbonate dehydratase I
  • carbonic anhydrase 1
  • Carbonic anhydrase B
  • Carbonic Anhydrase I
  • carbonic anhydrase ICAB
  • carbonic dehydratase
  • EC 4.2.1.1

Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CA1
Entrez
OMIM
Uniprot